Pancreatitis-Induced Depletion of Syntaxin 2 Promotes Autophagy and Increases Basolateral Exocytosis.

Autor:	Dolai S; Department of Medicine, University of Toronto, Toronto, Ontario, Canada. Electronic address: subhankar.dolai@gmail.com., Liang T; Department of Medicine, University of Toronto, Toronto, Ontario, Canada., Orabi AI; Children's Hospital of Pittsburgh of University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania., Holmyard D; Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, Canada., Xie L; Department of Medicine, University of Toronto, Toronto, Ontario, Canada., Greitzer-Antes D; Department of Medicine, University of Toronto, Toronto, Ontario, Canada., Kang Y; Department of Medicine, University of Toronto, Toronto, Ontario, Canada., Xie H; Department of Medicine, University of Toronto, Toronto, Ontario, Canada., Javed TA; Children's Hospital of Pittsburgh of University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania., Lam PP; Department of Medicine, University of Toronto, Toronto, Ontario, Canada., Rubin DC; Division of Gastroenterology, Departments of Medicine, and Developmental Biology, Washington University School of Medicine, St Louis, Missouri., Thorn P; University of Sydney, Sydney, New South Wales, Australia., Gaisano HY; Department of Medicine, University of Toronto, Toronto, Ontario, Canada; Department of Physiology, University of Toronto, Toronto, Ontario, Canada. Electronic address: herbert.gaisano@utoronto.ca.
Jazyk:	angličtina
Zdroj:	Gastroenterology [Gastroenterology] 2018 May; Vol. 154 (6), pp. 1805-1821.e5. Date of Electronic Publication: 2018 Jan 31.
DOI:	10.1053/j.gastro.2018.01.025
Abstrakt:	Background & Aims: Pancreatic acinar cells are polarized epithelial cells that store enzymes required for digestion as inactive zymogens, tightly packed at the cell apex. Stimulation of acinar cells causes the zymogen granules to fuse with the apical membrane, and the cells undergo exocytosis to release proteases into the intestinal lumen. Autophagy maintains homeostasis of pancreatic acini. Syntaxin 2 (STX2), an abundant soluble N-ethyl maleimide sensitive factor attachment protein receptor in pancreatic acini, has been reported to mediate apical exocytosis. Using human pancreatic tissues and STX2-knockout (KO) mice, we investigated the functions of STX2 in zymogen granule-mediated exocytosis and autophagy. Methods: We obtained pancreatic tissues from 5 patients undergoing surgery for pancreatic cancer and prepared 80-μm slices; tissues were exposed to supramaximal cholecystokinin octapeptide (CCK-8) or ethanol and a low concentration of CCK-8 and analyzed by immunoblot and immunofluorescence analyses. STX2-KO mice and syntaxin 2 +/+ C57BL6 mice (controls) were given intraperitoneal injections of supramaximal caerulein (a CCK-8 analogue) or fed ethanol and then given a low dose of caerulein to induce acute pancreatitis, or saline (controls); pancreata were isolated and analyzed by histology and immunohistochemistry. Acini were isolated from mice, incubated with CCK-8, and analyzed by immunofluorescence microscopy or used in immunoprecipitation experiments. Exocytosis was quantified using live-cell exocytosis and Ca 2+ imaging analyses and based on formation of exocytotic soluble N-ethyl maleimide sensitive factor attachment protein receptor complexes. Dysregulations in autophagy were identified using markers, electron and immunofluorescence microscopy, and protease activation assays. Results: Human pancreatic tissues and dispersed pancreatic acini from control mice exposed to CCK-8 or ethanol plus CCK-8 were depleted of STX2. STX2-KO developed more severe pancreatitis after administration of supramaximal caerulein or a 6-week ethanol diet compared with control. Acini from STX2-KO mice had increased apical exocytosis after exposure to CCK-8, as well as increased basolateral exocytosis, which led to ectopic release of proteases. These increases in apical and basolateral exocytosis required increased formation of fusogenic soluble N-ethyl maleimide sensitive factor attachment protein receptor complexes, mediated by STX3 and STX4. STX2 bound ATG16L1 and prevented it from binding clathrin. Deletion of STX2 from acini increased binding of AT16L1 to clathrin, increasing formation of pre-autophagosomes and inducing autophagy. Induction of autophagy promoted the CCK-8-induced increase in autolysosome formation and the activation of trypsinogen. Conclusions: In studies of human pancreatic tissues and pancreata from STX2-KO and control mice, we found STX2 to block STX3- and STX4-mediated fusion of zymogen granules with the plasma membrane and exocytosis and prevent binding of ATG16L1 to clathrin, which contributes to induction of autophagy. Exposure of pancreatic tissues to CCK-8 or ethanol depletes acinar cells of STX2, increasing basolateral exocytosis and promoting autophagy induction, leading to activation of trypsinogen. (Copyright © 2018 AGA Institute. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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