Triple S-Phase Labeling of Dividing Stem Cells.

Autor:	Podgorny O; Center for Developmental Genetics, Stony Brook University, Stony Brook, NY 11794, USA; Department of Anesthesiology, Stony Brook University, Stony Brook, NY 11794, USA; Moscow Institute of Physics and Technology, Moscow 123182, Russian Federation; Koltzov Institute of Developmental Biology RAS, Moscow 119334, Russian Federation., Peunova N; Center for Developmental Genetics, Stony Brook University, Stony Brook, NY 11794, USA; Department of Anesthesiology, Stony Brook University, Stony Brook, NY 11794, USA; Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA., Park JH; Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA., Enikolopov G; Center for Developmental Genetics, Stony Brook University, Stony Brook, NY 11794, USA; Department of Anesthesiology, Stony Brook University, Stony Brook, NY 11794, USA; Moscow Institute of Physics and Technology, Moscow 123182, Russian Federation; Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA. Electronic address: grigori.enikolopov@stonybrookmedicine.edu.
Jazyk:	angličtina
Zdroj:	Stem cell reports [Stem Cell Reports] 2018 Feb 13; Vol. 10 (2), pp. 615-626.
DOI:	10.1016/j.stemcr.2017.12.020
Abstrakt:	Marking replicating DNA with multiple labels presents the possibility of revealing new features and mechanisms of DNA synthesis and cell division; however, progression beyond double labeling has been hampered by cross-reactivity of label detection and scarcity of appropriate labels. Here, we present a method for triple S-phase labeling of the dividing cells, with a fourth label used to mark cells actively engaged in cell-cycle progression (e.g., using Ki67) or to phenotype the dividing cells or their progeny (e.g., using a GFP-expressing lineage reporter transgene). We apply this method to determine the parameters of neural stem cell division in the adult brain, to birth date up to four cohorts of dividing cells, and to reveal patterns of stem cell division in non-neural tissues. (Copyright © 2018 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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