Direct anti-inflammatory effects of granulocyte colony-stimulating factor (G-CSF) on activation and functional properties of human T cell subpopulations in vitro.

Autor: Malashchenko VV; Immanuel Kant Baltic Federal University, 14 A. Nevskogo St., Kaliningrad 236016, Russia. Electronic address: vvslon@rambler.ru., Meniailo ME; Immanuel Kant Baltic Federal University, 14 A. Nevskogo St., Kaliningrad 236016, Russia. Electronic address: max89me@yandex.ru., Shmarov VA; Immanuel Kant Baltic Federal University, 14 A. Nevskogo St., Kaliningrad 236016, Russia. Electronic address: enant@list.ru., Gazatova ND; Immanuel Kant Baltic Federal University, 14 A. Nevskogo St., Kaliningrad 236016, Russia. Electronic address: n_gazatova@mail.ru., Melashchenko OB; Immanuel Kant Baltic Federal University, 14 A. Nevskogo St., Kaliningrad 236016, Russia. Electronic address: omelashchenko@kantiana.ru., Goncharov AG; Immanuel Kant Baltic Federal University, 14 A. Nevskogo St., Kaliningrad 236016, Russia. Electronic address: agoncharov59@mail.ru., Seledtsova GV; Scientific Research Institute of Fundamental and Clinical Immunology, Novosibirsk 630099, Russia. Electronic address: galina-seledtsova@yandex.ru., Seledtsov VI; Immanuel Kant Baltic Federal University, 14 A. Nevskogo St., Kaliningrad 236016, Russia. Electronic address: seledtsov@rambler.ru.
Jazyk: angličtina
Zdroj: Cellular immunology [Cell Immunol] 2018 Mar; Vol. 325, pp. 23-32. Date of Electronic Publication: 2018 Jan 19.
DOI: 10.1016/j.cellimm.2018.01.007
Abstrakt: We investigated the direct effects of human granulocyte colony-stimulating factor (G-CSF) on functionality of human T-cell subsets. CD3 + T-lymphocytes were isolated from blood of healthy donors by positive magnetic separation. T cell activation with particles conjugated with antibodies (Abs) to human CD3, CD28 and CD2 molecules increased the proportion of cells expressing G-CSF receptor (G-CSFR, CD114) in all T cell subpopulations studied (CD45RA + /CD197 + naive T cells, CD45RA - /CD197 + central memory T cells, CD45RA - /CD197 - effector memory T cells and CD45RA + /CD197 - terminally differentiated effector T cells). Upon T-cell activation in vitro, G-CSF (10.0 ng/ml) significantly and specifically enhanced the proportion of CD114 + T cells in central memory CD4 + T cell compartment. A dilution series of G-CSF (range, 0.1-10.0 ng/ml) was tested, with no effect on the expression of CD25 (interleukin-2 receptor α-chain) on activated T cells. Meanwhile, G-CSF treatment enhanced the proportion of CD38 + T cells in CD4 + naïve T cell, effector memory T cell and terminally differentiated effector T cell subsets, as well as in CD4 - central memory T cells and terminally differentiated effector T cells. G-CSF did not affect IL-2 production by T cells; relatively low concentrations of G-CSF down-regulated INF-γ production, while high concentrations of this cytokine up-regulated IL-4 production in activated T cells. The data obtained suggests that G-CSF could play a significant role both in preventing the development of excessive and potentially damaging inflammatory reactivity, and in constraining the expansion of potentially cytodestructive T cells.
(Copyright © 2018 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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