Evaluating new bio-hydrogen producers: Clostridium perfringens strain JJC, Clostridium bifermentans strain WYM and Clostridium sp. strain Ade.TY.

Autor: Wong YM; Laboratory of Advance Catalysis and Environmental Technology, School of Science, Monash University, Jalan Lagoon Selatan, 46150 Bandar Sunway, Malaysia., Wu TY; Chemical and Sustainable Process Engineering Research Group, School of Engineering, Monash University, Jalan Lagoon Selatan, 46150 Bandar Sunway, Malaysia., Ling TC; Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia., Show PL; Department of Chemical and Environmental Engineering, Faculty of Engineering, University of Nottingham Malaysia Campus, Jalan Broga, 43500 Semenyih, Malaysia., Lee SY; Department of Chemical and Environmental Engineering, Faculty of Engineering, University of Nottingham Malaysia Campus, Jalan Broga, 43500 Semenyih, Malaysia., Chang JS; Research Center for Energy Technology and Strategy, National Cheng Kung University, Tainan 701, Taiwan., Ibrahim S; Department of Civil Engineering, Faculty of Engineering, University of Malaya, 50603 Kuala Lumpur, Malaysia., Juan JC; Laboratory of Advance Catalysis and Environmental Technology, School of Science, Monash University, Jalan Lagoon Selatan, 46150 Bandar Sunway, Malaysia; Nanotechnology and Catalysis Research Centre (NANOCAT), University of Malaya, 50603 Kuala Lumpur, Malaysia. Electronic address: jcjuan@um.edu.my.
Jazyk: angličtina
Zdroj: Journal of bioscience and bioengineering [J Biosci Bioeng] 2018 May; Vol. 125 (5), pp. 590-598. Date of Electronic Publication: 2018 Jan 17.
DOI: 10.1016/j.jbiosc.2017.12.012
Abstrakt: Three newly discovered H 2 producing bacteria namely Clostridium perfringens strain JJC, Clostridium bifermentans strain WYM and Clostridium sp. strain Ade.TY originated from landfill leachate sludge have demonstrated highly efficient H 2 production. The maximum H 2 production attained from these isolates are in the descending order of strain C. perfringens strain JJC > C. bifermentans strain WYM > Clostridium sp. strain Ade.TY with yield of 4.68 ± 0.12, 3.29 ± 0.11, and 2.87 ± 0.10 mol H 2 /mol glucose, respectively. The result has broken the conventional theoretical yield of 4 mol H 2 /mol glucose. These isolates were thermodynamically favourable with Gibbs free energy between -33 and -35 kJ/mol (under process conditions: pH 6, 37 °C and 5 g/L glucose). All three isolates favour butyrate pathway for H 2 production with the ratio of acetate and butyrate of 0.77, 0.65 and 0.80 for strain JJC, WYM and Ade.TY, respectively. This study reported provides a new insight on the potential of unique bacteria in H 2 production.
(Copyright © 2017 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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