| Autor: |
Moore EL, Arvidson R, Banks C, Urenda JP, Duong E, Mohammed H, Adams ME |
| Jazyk: |
angličtina |
| Zdroj: |
Biochemistry [Biochemistry] 2018 Mar 27; Vol. 57 (12), pp. 1907-1916. Date of Electronic Publication: 2018 Mar 12. |
| DOI: |
10.1021/acs.biochem.7b00916 |
| Abstrakt: |
The parasitoid wasp Ampulex compressa injects venom directly into the brain and subesophageal ganglion of the cockroach Periplaneta americana, inducing a 7 to 10 day lethargy termed hypokinesia. Hypokinesia presents as a significant reduction in both escape response and spontaneous walking. We examined aminergic and peptidergic components of milked venom with HPLC and MALDI-TOF mass spectrometry. HPLC coupled with electrochemical detection confirmed the presence of dopamine in milked venom, while mass spectrometry revealed that the venom gland and venom sac have distinct peptide profiles, with milked venom predominantly composed of venom sac peptides. We isolated and characterized novel α-helical, amphipathic venom sac peptides that constitute a new family of venom toxins termed ampulexins. Injection of the most abundant venom peptide, ampulexin 1, into the subesophageal ganglion of cockroaches resulted in a short-term increase in escape threshold. Neither milked venom nor venom peptides interfered with growth of Escherichia coli or Bacillus thuringiensis on agar plates, and exposure to ampulexins or milked venom did not induce cell death in Chinese hamster ovary cells (CHO-K1) or Hi5 cells ( Trichoplusia ni). |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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