The impact of preapheresis white blood cell count on autologous peripheral blood stem cell collection efficiency and HSC infusion side effect rate.

Autor: Sakashita AM; Hemotherapy and Cellular Therapy Department, Hospital Israelita Albert Einstein, Sao Paulo, SP, Brazil., Kondo AT; Hemotherapy and Cellular Therapy Department, Hospital Israelita Albert Einstein, Sao Paulo, SP, Brazil., Yokoyama APH; Hemotherapy and Cellular Therapy Department, Hospital Israelita Albert Einstein, Sao Paulo, SP, Brazil., Lira SMC; Hemotherapy and Cellular Therapy Department, Hospital Israelita Albert Einstein, Sao Paulo, SP, Brazil., Bub CB; Hemotherapy and Cellular Therapy Department, Hospital Israelita Albert Einstein, Sao Paulo, SP, Brazil., Souza AM; Hemotherapy and Cellular Therapy Department, Hospital Israelita Albert Einstein, Sao Paulo, SP, Brazil., Cipolletta ANF; Hemotherapy and Cellular Therapy Department, Hospital Israelita Albert Einstein, Sao Paulo, SP, Brazil., Alvarez KC; Hemotherapy and Cellular Therapy Department, Hospital Israelita Albert Einstein, Sao Paulo, SP, Brazil., Hamerschlak N; Hematology and Bone Marrow Transplant Unit, Hospital Israelita Albert Einstein, Sao Paulo, SP, Brazil., Kutner JM; Hemotherapy and Cellular Therapy Department, Hospital Israelita Albert Einstein, Sao Paulo, SP, Brazil., Chiattone CS; Hematology Department - Faculdade de Ciências Médicas da Santa Casa de São Paulo, Sao Paulo, SP, Brazil.
Jazyk: angličtina
Zdroj: Journal of clinical apheresis [J Clin Apher] 2018 Jun; Vol. 33 (3), pp. 331-341. Date of Electronic Publication: 2018 Jan 19.
DOI: 10.1002/jca.21614
Abstrakt: Background: Autologous peripheral blood hematopoietic stem cell (PBSC) collection efficiency (CE) is reportedly affected by the patient's blood properties; however, studies to identify factors correlated with CE have shown inconsistent results. Additionally, variables such as stem cell graft granulocyte content and patient age, sex, and underlying disease, may be associated with hematopietic stem cell (HSC) infusion-related adverse reactions. In this study, we evaluated the correlation of preleukapheresis PB granulocyte count and PBSC harvest variables with CD34 + collection yield and efficiency, and thawed HSC infusion side effect occurrence.
Patients and Methods: We evaluated data from 361 patients who had undergone autologous PBSC transplant. Large volume leukapheresis was the method for PBSC collection. Complete Blood Count and CD34 + cell enumeration were performed in the preapheresis PB and the apheresis product sample. The PBSC grafts were submitted to non-controlled rate freezing after addition of 5% DMSO plus 6% hidroxyethylstarch as a cryoprotectant solution. The cryopreserved graft was thawed in a 37°C water bath and then infused without further manipulation.
Results: The CD34 + yield was associated with preapheresis PB CD34 + count and immature granulocyte count. The PBSC CE was negatively correlated with preapheresis white blood cell (WBC), immature granulocyte and granulocyte count. The leukapheresis product total nucleated cell (TNC) and granulocyte content was correlated with the thawed graft infusion side effect occurrence.
Conclusion: This study has shown that preapheresis PB WBC and granulocyte counts were associated with leukapheresis CE. Additionally, the leukapheresis product TNC and granulocyte content was correlated with thawed graft infusion side effect occurrence.
(© 2018 Wiley Periodicals, Inc.)
Databáze: MEDLINE
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