Motile Salmonella serotypes causing high mortality in poultry farms in three South-Western States of Nigeria.

Autor: Mshelbwala FM; Department of Veterinary Pathology, Federal University of Agriculture, Abeokuta, Nigeria., Ibrahim ND; Department of Veterinary Pathology, Ahmadu Bello University, Zaria, Nigeria., Saidu SN; Department of Veterinary Medicine, Ahmadu Bello University, Zaria, Nigeria., Azeez AA; Department of Veterinary Pathology, Federal University of Agriculture, Abeokuta, Nigeria., Akinduti PA; Department of Veterinary Microbiology and Parasitology, Federal University of Agriculture, Abeokuta, Nigeria., Kwanashie CN; Department of Veterinary Microbiology, Ahmadu Bello University, Zaria, Nigeria., Fakilahyel Kadiri AK; Department of Veterinary Medicine, Ahmadu Bello University, Zaria, Nigeria., Muhammed M; Department of Bacteriology, Salmonella Research Division, National Veterinary Research Instutute, Jos, Plateau State, Nigeria., Fagbamila IO; Department of Bacteriology, Salmonella Research Division, National Veterinary Research Instutute, Jos, Plateau State, Nigeria., Luka PD; Department of Biotechnology, National Veterinary Research Institute, Jos, Plateau State, Nigeria.
Jazyk: angličtina
Zdroj: Veterinary record open [Vet Rec Open] 2017 Dec 22; Vol. 4 (1), pp. e000247. Date of Electronic Publication: 2017 Dec 22 (Print Publication: 2017).
DOI: 10.1136/vetreco-2017-000247
Abstrakt: This study was carried out to identify the Salmonella serotypes causing high mortality in chickens in Lagos, Ogun and Oyo states, Nigeria. Chickens presented for postmortem examination during disease outbreaks that were characterised by high mortality (40 per cent to 80 per cent) in poultry farms in the study area were examined from January to December, 2013. Samples of the lungs, heart, liver, spleen, kidneys, proventriculus, intestine and caecum were collected from suspected cases of salmonellosis, for bacterial culture and identification. Salmonella isolates were confirmed using PCR and serotyped using the Kauffman-White scheme. Twenty-six day-old pullets were raised to two weeks and inoculated orally with 0.2 mL of 1×10 8  colony forming units of Salmonella Zega identified in the present study to determine their pathogenicity, while another 26 served as control. The Salmonella serotypes were S Zega (n=13; 35.14 per cent), Salmonella Kentucky (n=9; 24.32 per cent), Salmonella Herston (n=6; 16.22 per cent), Salmonella Nima (n=4; 10.81 per cent), Salmonella Telelkebir (n=3; 8.11 per cent), Salmonella Colindale (n=1; 2.70 per cent) and Salmonella Tshiongwe (n=1; 2.70 per cent). Clinical signs in both natural and experimental infections were acute (70 per cent) and chronic (30 per cent), and included weakness, anorexia, yellowish diarrhoea, pasted vents, somnolescence and mortality, while gross lesions showed marked pulmonary congestion and oedema, necrotic foci in the myocardium; the liver, spleen and kidneys were markedly enlarged and had subcapsular multifocal necrosis. There were catarrhal proventriculitis and enteritis, and haemorrhagic typhlitis. While most of the serotypes identified in the present study have been isolated from poultry sources from commercial farms in Nigeria, to the best of the authors' knowledge, they have not been previously reported to cause high mortality in chickens in the study area.
Competing Interests: Competing interests: None declared.
Databáze: MEDLINE
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