VascuTrainer: A Mobile and Disposable Bioreactor System for the Conditioning of Tissue-Engineered Vascular Grafts.

Autor: Wolf F; Department of Biohybrid & Medical Textiles (BioTex), AME-Helmholtz Institute for Biomedical Engineering & Institut für Textiltechnik (ITA), RWTH Aachen University, Pauwelsstraße 20, 52074, Aachen, Germany.; AMIBM-Aachen-Maastricht-Institute for Biobased Materials, Maastricht University, Maastricht, The Netherlands., Rojas González DM; Department of Biohybrid & Medical Textiles (BioTex), AME-Helmholtz Institute for Biomedical Engineering & Institut für Textiltechnik (ITA), RWTH Aachen University, Pauwelsstraße 20, 52074, Aachen, Germany.; AMIBM-Aachen-Maastricht-Institute for Biobased Materials, Maastricht University, Maastricht, The Netherlands., Steinseifer U; Institute of Applied Medical Engineering, Helmholtz Institute, RWTH Aachen University, Pauwelsstraße 20, 52074, Aachen, Germany., Obdenbusch M; Laboratory of Machine Tools and Production Engineering, Department of Automation & Control, RWTH Aachen University, Steinbachstraße 19, 52074, Aachen, Germany., Herfs W; Laboratory of Machine Tools and Production Engineering, Department of Automation & Control, RWTH Aachen University, Steinbachstraße 19, 52074, Aachen, Germany., Brecher C; Laboratory of Machine Tools and Production Engineering, Department of Automation & Control, RWTH Aachen University, Steinbachstraße 19, 52074, Aachen, Germany., Jockenhoevel S; Department of Biohybrid & Medical Textiles (BioTex), AME-Helmholtz Institute for Biomedical Engineering & Institut für Textiltechnik (ITA), RWTH Aachen University, Pauwelsstraße 20, 52074, Aachen, Germany.; AMIBM-Aachen-Maastricht-Institute for Biobased Materials, Maastricht University, Maastricht, The Netherlands., Mela P; Department of Biohybrid & Medical Textiles (BioTex), AME-Helmholtz Institute for Biomedical Engineering & Institut für Textiltechnik (ITA), RWTH Aachen University, Pauwelsstraße 20, 52074, Aachen, Germany. mela@ame.rwth-aachen.de.; AMIBM-Aachen-Maastricht-Institute for Biobased Materials, Maastricht University, Maastricht, The Netherlands. mela@ame.rwth-aachen.de., Schmitz-Rode T; Institute of Applied Medical Engineering, Helmholtz Institute, RWTH Aachen University, Pauwelsstraße 20, 52074, Aachen, Germany.
Jazyk: angličtina
Zdroj: Annals of biomedical engineering [Ann Biomed Eng] 2018 Apr; Vol. 46 (4), pp. 616-626. Date of Electronic Publication: 2018 Jan 16.
DOI: 10.1007/s10439-018-1977-y
Abstrakt: In vitro tissue engineering of vascular grafts requires dynamic conditioning in a bioreactor system for in vitro tissue maturation and remodeling to receive a mechanically adequate and hemocompatible implant. The goal of the current work was to develop a bioreactor system for the conditioning of vascular grafts which is (i) able to create a wide range of flow, pressure and frequency conditions, including physiological ones; (ii) compact and easy to assemble; (iii) transportable; (iv) disposable. The system is driven by a small centrifugal pump controlled via a custom-made control unit, which can also be operated on batteries to allow for autonomous transportation. To show the potential of the newly developed bioreactor system small-caliber vascular composite grafts (n = 5, internal diameter = 3 mm, length = 12.5 cm) were fabricated using a fibrin scaffold embedding human umbilical artery smooth muscle cells and a polyvinylidene fluoride warp-knitted macroporous mesh. Subsequently, the vascular grafts were endothelialized and mounted in the bioreactor system for conditioning. The conditioning parameters remained within the predefined range over the complete conditioning period and during operation on batteries as tested for up to 25 h. Fabrication and pre-conditioning under arterial pressure and shear stress conditions resulted in robust and hemocompatible tissue-engineered vascular grafts. Analysis of immunohistochemical stainings against extracellular matrix and cell-specific proteins revealed collagen I and collagen III deposition. The luminal surface was confluently covered with endothelial cells. The developed bioreactor system showed cytocompatibility and pH, pO 2 , pCO 2 , glucose and lactate stayed constant. Sterility was maintained during the complete fabrication process of the vascular grafts. The potential of a versatile and mobile system and its functionality by conditioning tissue-engineered vascular grafts under physiological pressure and flow conditions could be demonstrated.
Databáze: MEDLINE
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