Carbon nanospheres mediated nuclear delivery of SMAR1 protein (DNA binding domain) controls breast tumor in mice model.
| Autor: | Bhagat PN; Agharkar Research Institute, G. G. Agarkar Road, Pune 411004, Maharashtra, India., Jadhav SH; Agharkar Research Institute, G. G. Agarkar Road, Pune 411004, Maharashtra, India., Chattopadhyay S; National Centre for Cell Science, S.P. Pune University Campus, Ganeshkhind Road, Pune 411007, Maharashtra, India., Paknikar KM; Agharkar Research Institute, G. G. Agarkar Road, Pune 411004, Maharashtra, India. |
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| Jazyk: | angličtina |
| Zdroj: | Nanomedicine (London, England) [Nanomedicine (Lond)] 2018 Feb; Vol. 13 (4), pp. 353-372. Date of Electronic Publication: 2018 Jan 17. |
| DOI: | 10.2217/nnm-2017-0298 |
| Abstrakt: | Aim: To investigate anticancer activity of the DNA binding domain of SMAR1 (His 5) in vitro and in vivo. Materials & Methods: His 5 was conjugated to hydrothermally synthesized carbon nanospheres (CNs). Anticancer activity of CNs-His 5 was evaluated in vitro and in vivo. Results: CNs- His 5 significantly reduced cyclin D1 levels in MDA-MB-231 cells. Tumor bearing Balb/c mice injected with CNs-His 5 showed approximately 62% tumor regression and significantly reduced 18 FDG uptake. Caspases assay and IHC staining confirmed tumor growth inhibition, which could be attributed to apoptotic, antiproliferative and antiangiogenic activities of His 5. Conclusion: DNA binding domain of the SMAR1 protein (His 5) has potent anticancer activity and its CNs mediated delivery could control breast tumor in mice model. |
| Databáze: | MEDLINE |
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