Could cryopreserved human semen samples be stored at -80°C?

Autor:	Vaz CR; Laboratório de Biotecnologia da Reprodução (LBR), Universidade do Vale do Itajaí (UNIVALI), Itajaí, Santa Catarina, Brazil., Lamim T; Laboratório de Biotecnologia da Reprodução (LBR), Universidade do Vale do Itajaí (UNIVALI), Itajaí, Santa Catarina, Brazil., Salvador RA; Laboratório de Biotecnologia da Reprodução (LBR), Universidade do Vale do Itajaí (UNIVALI), Itajaí, Santa Catarina, Brazil., Batschauer APB; Laboratório de Biotecnologia da Reprodução (LBR), Universidade do Vale do Itajaí (UNIVALI), Itajaí, Santa Catarina, Brazil., Amaral VLL; Laboratório de Biotecnologia da Reprodução (LBR), Universidade do Vale do Itajaí (UNIVALI), Itajaí, Santa Catarina, Brazil., Til D; Laboratório de Biotecnologia da Reprodução (LBR), Universidade do Vale do Itajaí (UNIVALI), Itajaí, Santa Catarina, Brazil.
Jazyk:	angličtina
Zdroj:	JBRA assisted reproduction [JBRA Assist Reprod] 2018 Jun 01; Vol. 22 (2), pp. 108-112. Date of Electronic Publication: 2018 Jun 01.
DOI:	10.5935/1518-0557.20180016
Abstrakt:	Objective: To evaluate storage time effects in cryopreserved human semen samples, kept in the freezer at a controlled temperature of -80°C, on sperm viability after thawing. Methods: We used 20 semen samples. Each sample was cryopreserved in 10 fingers, which were divided into five groups: one group was kept in cryogenic canisters throughout the experiment(control), and four groups were kept in a VIP Ultra Low MDF-U76V- PE freezer, with the temperature set at -80°C, for 24, 48, 72 and 96 hours, respectively. After the exposure time, the samples were stored in cryogenic canisters after being thawed. The analyzed parameters were: motility, vitality and mitochondrial activity. Results: After thawing, we noticed decreased sperm motility, vitality and mitochondrial activity, when comparing the tested groups with the control group, as well as a progressive reduction in the analyzed parameters between the times evaluated. Conclusions: Cryopreservation of semen samples at -80°C is potentially harmful to sperm viability, causing damage when submitted to longer exposure times.
Databáze:	MEDLINE
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