Autor: |
Thammasittirong SN; Department of Founding Project of Microbiology, Faculty of Liberal Arts and Science, Kasetsart University Kampheang Saen Campus, Nakorn Pathom, Thailand., Chamduang T; Department of Founding Project of Microbiology, Faculty of Liberal Arts and Science, Kasetsart University Kampheang Saen Campus, Nakorn Pathom, Thailand., Phonrod U; Department of Science, Faculty of Liberal Arts and Science, Kasetsart University Kamphaeng Sean Campus, Nakorn Pathom, Thailand., Sriroth K; Department of Biotechnology, Faculty of Agro-industry, Kasetsart University, Bangkok, Thailand. |
Abstrakt: |
Four ethanologenic ethanol-tolerant yeast strains, Saccharomyces cerevisiae (ATKU132), Saccharomycodes ludwigii (ATKU47), and Issatchenkia orientalis (ATKU5-60 and ATKU5-70), were isolated by an enrichment technique in yeast extract peptone dextrose (YPD) medium supplemented with 10% (v/v) ethanol at 30°C. Among non-Saccharomyces yeasts, Sd. ludwigii ATKU47 exhibited the highest ethanol-tolerance and ethanol production, which was similar to S. cerevisiae ATKU132. The maximum range of ethanol concentrations produced at 37°C by S. cerevisiae ATKU132 and Sd. ludwigii ATKU47 from an initial D-glucose concentration of 20% (w/v) and 28% (w/v) sugarcane molasses were 9.46-9.82% (w/v) and 8.07-8.32% (w/v), respectively. |