Preventive effects of Salvia officinalis leaf extract on insulin resistance and inflammation in a model of high fat diet-induced obesity in mice that responds to rosiglitazone.

Autor:	Ben Khedher MR; Biochemistry Department, Research Laboratory 'Nutrition-Functional Food & Vascular Health', Faculty of Medicine, University of Monastir, Monastir, Tunisia., Hammami M; Biochemistry Department, Research Laboratory 'Nutrition-Functional Food & Vascular Health', Faculty of Medicine, University of Monastir, Monastir, Tunisia., Arch JRS; Buckingham Institute for Translational Medicine (BITM), Clore Laboratory, University of Buckingham, Buckingham, United Kingdom., Hislop DC; Buckingham Institute for Translational Medicine (BITM), Clore Laboratory, University of Buckingham, Buckingham, United Kingdom., Eze D; Medical School, University of Buckingham, Buckingham, United Kingdom., Wargent ET; Buckingham Institute for Translational Medicine (BITM), Clore Laboratory, University of Buckingham, Buckingham, United Kingdom., Kępczyńska MA; Buckingham Institute for Translational Medicine (BITM), Clore Laboratory, University of Buckingham, Buckingham, United Kingdom., Zaibi MS; Buckingham Institute for Translational Medicine (BITM), Clore Laboratory, University of Buckingham, Buckingham, United Kingdom.
Jazyk:	angličtina
Zdroj:	PeerJ [PeerJ] 2018 Jan 09; Vol. 6, pp. e4166. Date of Electronic Publication: 2018 Jan 09 (Print Publication: 2018).
DOI:	10.7717/peerj.4166
Abstrakt:	Background: Salvia officinalis (sage) is a native plant to the Mediterranean region and has been used for a long time in traditional medicine for various diseases. We investigated possible anti-diabetic, anti-inflammatory and anti-obesity effects of sage methanol (MetOH) extract in a nutritional mouse model of obesity, inflammation and insulin resistance, as well as its effects on lipolysis and lipogenesis in 3T3-L1 cells. Methods: Diet-induced obese (DIO) mice were treated for five weeks with sage methanol extract (100 and 400 mg kg -1 /day bid), or rosiglitazone (3 mg kg -1 /day bid), as a positive control. Energy expenditure, food intake, body weight, fat mass, liver glycogen and lipid content were evaluated. Blood glucose, and plasma levels of insulin, lipids leptin and pro- and anti-inflammatory cytokines were measured throughout the experiment. The effects of sage MetOH extract on lipolysis and lipogenesis were tested in vitro in 3T3-L1 cells. Results: After two weeks of treatment, the lower dose of sage MetOH extract decreased blood glucose and plasma insulin levels during an oral glucose tolerance test (OGTT). An insulin tolerance test (ITT), performed at day 29 confirmed that sage improved insulin sensitivity. Groups treated with low dose sage and rosiglitazone showed very similar effects on OGTT and ITT. Sage also improved HOMA-IR, triglycerides and NEFA. Treatment with the low dose increased the plasma levels of the anti-inflammatory cytokines IL-2, IL-4 and IL-10 and reduced the plasma level of the pro-inflammatory cytokines IL-12, TNF-α, and KC/GRO. The GC analysis revealed the presence of two PPARs agonist in sage MetOH extract. In vitro , the extract reduced in a dose-related manner the accumulation of lipid droplets; however no effect on lipolysis was observed. Conclusions: Sage MetOH extract at low dose exhibits similar effects to rosiglitazone. It improves insulin sensitivity, inhibits lipogenesis in adipocytes and reduces inflammation as judged by plasma cytokines. Sage presents an alternative to pharmaceuticals for the treatment of diabetes and associated inflammation. Competing Interests: The authors declare there are no competing interests.
Databáze:	MEDLINE
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