| Autor: |
Malami I; Faculty of Pharmaceutical Sciences, Usmanu Danfodiyo University, Sokoto, Nigeria., Muhammad A; Department of Biochemistry, Faculty of Life Sciences, Ahmadu Bello University, Zaria, Nigeria., Etti IC; Department of Pharmacology and Toxicology, Universiti of Uyo, Uyo, Nigeria., Waziri PM; Department of Biochemistry, Kaduna State University, Kaduna, Nigeria., Alhassan AM; Faculty of Pharmaceutical Sciences, Usmanu Danfodiyo University, Sokoto, Nigeria. |
| Jazyk: |
angličtina |
| Zdroj: |
EXCLI journal [EXCLI J] 2017 Dec 08; Vol. 16, pp. 1276-1287. Date of Electronic Publication: 2017 Dec 08 (Print Publication: 2017). |
| DOI: |
10.17179/excli2017-299 |
| Abstrakt: |
R273H mutant p53 is a DNA-contact mutant that renders p53 dysfunctional due to a single substitution of Arg273 for His273. Rescuing R273 mutant p53 implies that a competent molecule would have to bind to the site of DNA-contact hot spots to complement the loss of contact with the DNA-binding domain. Here, curcumin, flavokawain B, and alpinetin were docked against the crystal structure of R273H mutant p53 in silico . Consequently, all the compounds bind to the cavity of R273H mutant p53 with a dissociation constant estimated to have 36.57, 70.77, and 75.11 µM for curcumin, flavokawain B, and alpinetin, respectively. Subsequently, each molecule was able to bind to the R273H mutant p53 by interacting with the DNA-contact hot spot Arg248 and mutant R273H, thereby compensating for the loss of direct contact with the DNA-binding domain. Furthermore, all the molecules were able to induce a direct contact with the consensus site of the DNA binding domain, thus maintaining DNA-contact residues with the DNA. The present findings offer preliminary indirect supporting evidence that small molecular weight compounds may certainly rescue DNA-contact mutant p53, which may lay a foundation for designing a competent and effective molecule capable of rescuing mutant p53 in tumor cells expressing R273H mutant p53. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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