Ultrastructural damage in Streptococcus mutans incubated with saliva and histatin 5.

Autor: Fernández-Presas AM; Departamento de Microbiología y Parasitología, Facultad de Medicina, Av. Universidad 3000, Col. Universidad Nacional Autónoma de México, CP 04510, Mexico City, México. Electronic address: presas@unam.mx., Márquez Torres Y; Departamento de Microbiología y Parasitología, Facultad de Medicina, Av. Universidad 3000, Col. Universidad Nacional Autónoma de México, CP 04510, Mexico City, México., García González R; Departamento de Microbiología y Parasitología, Facultad de Medicina, Av. Universidad 3000, Col. Universidad Nacional Autónoma de México, CP 04510, Mexico City, México., Reyes Torres A; Departamento de Microbiología y Parasitología, Facultad de Medicina, Av. Universidad 3000, Col. Universidad Nacional Autónoma de México, CP 04510, Mexico City, México., Becker Fauser I; Unidad de Investigación en Medicina Experimental, Universidad Nacional Autónoma de México, Hospital General de México, Dr. Balmis, 148 Col. Doctores, Del. Cuauhtémoc, C.P. 06726, Mexico City, Mexico., Rodríguez Barrera H; Departamento de Microbiología y Parasitología, Facultad de Medicina, Av. Universidad 3000, Col. Universidad Nacional Autónoma de México, CP 04510, Mexico City, México., Ruíz García B; Departamento de Microbiología y Parasitología, Facultad de Medicina, Av. Universidad 3000, Col. Universidad Nacional Autónoma de México, CP 04510, Mexico City, México., Toloza Medina R; Departamento de Microbiología y Parasitología, Facultad de Medicina, Av. Universidad 3000, Col. Universidad Nacional Autónoma de México, CP 04510, Mexico City, México., Delgado Domínguez J; Unidad de Investigación en Medicina Experimental, Universidad Nacional Autónoma de México, Hospital General de México, Dr. Balmis, 148 Col. Doctores, Del. Cuauhtémoc, C.P. 06726, Mexico City, Mexico., Molinarí Soriano JL; Departamento de Bioquímica y Biología Estructural, Instituto de Fisiología Celular, Av. Universidad 3000, Col. Universidad Nacional Autónoma de México, CP 04510, México City, Mexico.
Jazyk: angličtina
Zdroj: Archives of oral biology [Arch Oral Biol] 2018 Mar; Vol. 87, pp. 226-234. Date of Electronic Publication: 2018 Jan 08.
DOI: 10.1016/j.archoralbio.2018.01.004
Abstrakt: Objective: To study the ultrastructural alterations induced in Streptococcus mutans (ATCC 25175) incubated with saliva, saliva plus histatin 5 and histatin 5.
Methods: S. mutans incubated with saliva histatin 5 or a combination of both were morphologically analyzed and counted. The results were expressed as (CFU)ml -1 . Ultrastructural damage was evaluated by transmission electron microscopy. Ultrastructural localization of histatin 5 was examined using immunogold labeling. Apoptotic cell death was determined by flow cytometry (TUNEL).
Results: A decrease in the bacteria numbers was observed after incubation with saliva, saliva with histatin 5 or histatin 5 compared to the control group (p<0.0001). Ultrastructural damage in S. mutans incubated with saliva was found in the cell wall. Saliva plus histatin 5 induced a cytoplasmic granular pattern and decreased the distance between the plasma membrane bilayers, also found after incubation with histatin 5, together with pyknotic nucleoids. Histatin 5 was localized on the bacterial cell walls, plasma membranes, cytoplasm and nucleoids. Apoptosis was found in the bacteria incubated with saliva (63.9%), saliva plus histatin 5 (71.4%) and histatin 5 (29.3%). Apoptosis in the control bacteria was 0.2%.
Conclusions: Antibacterial activity against S. mutans and the morphological description of damage induced by saliva and histatin 5 was demonstrated. Pyknotic nucleoids observed in S. mutans exposed to saliva, saliva plus histatin 5 and histatin 5 could be an apoptosis-like death mechanism. The knowledge of the damage generated by histatin 5 and its intracellular localization could favor the design of an ideal peptide as a therapeutic agent.
(Copyright © 2018 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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