Low-intensity training provokes adaptive extracellular matrix turnover of a muscular dystrophy model.

Autor:	Gaiad TP; Department of Physical Therapy, Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM), Diamantina, Brazil., Oliveira MX; Department of Physical Therapy, Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM), Diamantina, Brazil., Lobo AR Jr; Institute of Agriculture Scinces, Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM), Unaí, Brazil., Libório LR; Department of Physical Therapy, Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM), Diamantina, Brazil., Pinto PAF; Department of Physical Therapy, Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM), Diamantina, Brazil., Fernandes DC; Department of Physical Therapy, Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM), Diamantina, Brazil., Santos AP; Department of Physical Therapy, Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM), Diamantina, Brazil., Ambrósio CE; Department of Veterinary Medicine, University of São Paulo (USP), Pirassununga, Brazil., Machado ASD; Faculty of Medicine FAMED, Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM), Diamantina, Brazil.
Jazyk:	angličtina
Zdroj:	Journal of exercise rehabilitation [J Exerc Rehabil] 2017 Dec 27; Vol. 13 (6), pp. 693-703. Date of Electronic Publication: 2017 Dec 27 (Print Publication: 2017).
DOI:	10.12965/jer.1735094.547
Abstrakt:	Recommendations of therapeutic exercise in Duchenne muscular dystrophy are still controversial. The hypothesis that a low-intensity training (LIT) protocol leads to muscle adaptations on mdx mice model was tested. Dystrophic male mice with 8 weeks old were separated in exercised (mdxE, n= 8) and sedentary (mdxC, n= 8) groups. Wild-type mice were used as control (WT, n= 8) group. Exercised group underwent a LIT protocol (9 m/min, 30 min, 3 days/wk, 60 days) on a horizontal treadmill. At day 60 all animals were analyzed regarding parameters of markers of muscle lesion and extracellular matrix turnover of muscle tissue by collagens fibers on tibial anterior muscle. Histomorphometry attested that centrally located nuclei fibers and the coefficient of variance of minimal Feret's diameter was similar in mdxE and mdxC groups ( P = 1.000) and both groups presented higher mean values than WT group ( P < 0.001). Fraction area of collagen fibers of mdxE group was lower than mdxC group ( P = 0,027) and similar to WT group ( P = 0,751). Intramuscular area of Col3 of the mdxE group was higher than mdxC and WT groups ( P <0.001). Intramuscular area of Col1 on the mdxE group was similar to the mdxC group ( P = 1.000) and both groups were higher than WT group ( P < 0.001). LIT protocol had not influenced muscle injuries resulting from the dystrophin-deficiency membrane fragility. Although, LIT had provoked adaptations on extracellular matrix bringing higher elastic feature to dystrophic muscle tissue. Competing Interests: CONFLICT OF INTEREST No potential conflict of interest relevant to this article was reported.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu