Comparison among bone marrow mesenchymal stem and mononuclear cells to promote functional recovery after spinal cord injury in rabbits.

Autor:	Fonseca AFB; PhD in Sciences, Laboratory of Animal Health, Center for Agricultural Sciences and Technologies, Animal Experimentation Unit, Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Campos dos Goytacazes-RJ, Brazil. Intellectual, scientific, conception and design of the study; technical procedures; aquisition and analysis of data; manuscript writing., Scheffer JP; Fellow PhD degree, Laboratory of Animal Health, Center for Agricultural Sciences and Technologies, Animal Experimentation Unit, UENF, Campos dos Goytacazes-RJ, Brazil. Conception and design of the estudy, technical procedures, critical revision., Giraldi-Guimarães A; PhD in Sciences, Associate Professor, Laboratory of Cell and Tissue Biology, Center for Biosciences and Biotechnology, UENF, Campos dos Goytacazes-RJ, Brazil. Analysis and interpretation of data., Coelho BP; PhD in Sciences, Laboratory of Cell and Tissue Biology, Center for Biosciences and Biotechnology, UENF, Campos dos Goytacazes-RJ, Brazil. Acquisition of data., Medina RM; PhD in Sciences, Laboratory of Animal Health; Center for Agricultural Sciences and Technologies, Animal Experimentation Unit, UENF, Campos dos Goytacazes-RJ, Brazil. Acquisition of data., Oliveira ALA; PhD in Sciences, Associate Professor, Laboratory of Animal Health; Center for Agricultural Sciences and Technologies, Animal Experimentation Unit, UENF, Campos dos Goytacazes-RJ, Brazil. Critical revision, final approval the manuscript.
Jazyk:	angličtina
Zdroj:	Acta cirurgica brasileira [Acta Cir Bras] 2017 Dec; Vol. 32 (12), pp. 1026-1035.
DOI:	10.1590/s0102-865020170120000004
Abstrakt:	Purpose: To investigate the efficacy of allogeneic mesenchymal stem-cells and autologous mononuclear cells to promote sensorimotor recovery and tissue rescue. Methods: Female rabbits were submitted to the epidural balloon inflation method and the intravenous cells administrations were made after 8 hours or seven days after injury induction. Sensorimotor evaluation of the hindlimbs was performed, and the euthanasia was made thirty days after the treatment. Spinal cords were stained with hematoxylin and eosin. Results: Both therapies given 8 hours after the injury promoted the sensorimotor recovery after a week. Only the group treated after a week with mononuclear cells showed no significant recovery at post-injury day 14. In the days 21 and 28, all treatments promoted significant recovery. Histopathological analysis showed no difference among the experimental groups. Our results showed that both bone marrow-derived cell types promoted significant sensorimotor recovery after injury, and the treatment made at least a week after injury is efficient. Conclusion: The possibilities of therapy with bone marrow-derived cells are large, increasing the therapeutic arsenal for the treatment of spinal cord injury.
Databáze:	MEDLINE
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