| Autor: |
Ribeiro AR; Department of Parasitology, Universidade Estadual de Campinas, Campinas, Brazil., Lima L; Department of Parasitology, Universidade de São Paulo, São Paulo, Brazil., de Almeida LA; Department of Biological Sciences, Faculdade de Ciências Farmacêuticas da Universidade Estadual Paulista Júlio de Mesquita Filho, Araraquara, Brazil., Monteiro J; Global Health and Tropical Medicine, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Lisbon, Portugal., Moreno CJG; Programa de Pós-graduação em Bioquímica, Departamento de Bioquímica, Centro de Biociência, Universidade Federal do Rio Grande do Norte, Natal, Brazil., Nascimento JD; Department of Parasitology, Universidade Estadual de Campinas, Campinas, Brazil., de Araújo RF; Bahia State Health Secretariat, Salvador, Brazil., Mello F; Rio Grande do Sul State Health Secretariat, Porto Alegre, Brazil., Martins LPA; Faculdade de Medicina de Marília, Marília, Brazil., Graminha MAS; Department of Biological Sciences, Faculdade de Ciências Farmacêuticas da Universidade Estadual Paulista Júlio de Mesquita Filho, Araraquara, Brazil., Teixeira MMG; Department of Parasitology, Universidade de São Paulo, São Paulo, Brazil., Silva MS; Departamento de Análises Clínicas e Toxicológicas, Centro de Ciências da Saúde, Universidade Federal do Rio Grande do Norte, Natal, Brazil.; Programa de Pós-graduação em Bioquímica, Departamento de Bioquímica, Centro de Biociência, Universidade Federal do Rio Grande do Norte, Natal, Brazil.; Global Health and Tropical Medicine, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Lisbon, Portugal., Steindel M; Department of Microbiology, Immunology, and Parasitology, Universidade Federal de Santa Catarina, Florianópolis, Brazil., da Rosa JA; Department of Biological Sciences, Faculdade de Ciências Farmacêuticas da Universidade Estadual Paulista Júlio de Mesquita Filho, Araraquara, Brazil. |
| Abstrakt: |
Chagas disease affects between six and seven million people. Its etiological agent, Trypanosoma cruzi , is classified into six discrete typing units (DTUs). The biological study of 11 T. cruzi strains presented here included four parameters: growth kinetics, parasitemia curves, rate of macrophage infection, and serology to evaluate IgM, total IgG, IgG1, IgG2a, and IgG3. Sequencing of small subunit of ribosomal RNA (SSU rRNA)was performed and the T. cruzi strains were classified into three DTUs. When their growth in liver infusion tryptose medium was represented in curves, differences among the strains could be noted. The parasitemia profile varied among the strains from the TcI, TcII, and TcIII groups, and the 11 T. cruzi strains produced distinct parasitemia levels in infected BALB/c. The TcI group presented the highest rate of macrophage infection by amastigotes, followed by TcII and TcIII. Reactivity to immunoglobulins was observed in the TcI, TcII, and TcIII; all the animals infected with the different strains of T. cruzi showed anti- T. cruzi antibodies. The molecular study presented here resulted in the classification of the T. cruzi strains into the TcI (Bolivia, T lenti, Tm, SC90); TcII (Famema, SC96, SI8, Y); and TcIII (QMM3, QMM5, SI5) groups. These biological and molecular results from 11 T. cruzi strains clarified the factors involved in the biology of the parasite and its hosts. The collection of triatomine (vector) species, and the study of geographic distribution, as well as biological and molecular characterization of the parasite, will contribute to the reporting and surveillance measures in Brazilian states. |
