Activation of NK Cells in Mixed Cultures of Wharton's Jelly Mesenchymal Stromal Cells and Peripheral Blood Lymphocytes.

Autor: Svirshchevskaya EV; M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia. esvir@mx.ibch.ru., Poltavtsev AM; Institute of Applied Mechanics, Russian Academy of Sciences, Moscow, Russia., Os'mak GZ; V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of the Health of the Russian Federation, Moscow, Russia., Poltavtseva RA; V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of the Health of the Russian Federation, Moscow, Russia.
Jazyk: angličtina
Zdroj: Bulletin of experimental biology and medicine [Bull Exp Biol Med] 2018 Jan; Vol. 164 (3), pp. 339-343. Date of Electronic Publication: 2018 Jan 08.
DOI: 10.1007/s10517-018-3985-1
Abstrakt: Mesenchymal stromal cells possess immunosuppressive properties that might be used for the therapy of inflammatory diseases of various geneses. The effects of mesenchymal stromal cells depend on their lifetime in the recipient tissues. During heterologous transplantation, mesenchymal stromal cells are eliminated by NK cells. We studied NK cell formation in mixed cultures of Wharton's jelly mesenchymal stromal cells and peripheral blood lymphocytes from an autologous donor. Lymphocytes were activated by a mitogen or IL-2. The lifetime of mesenchymal stromal cells was estimated by MTT test. Cytotoxic activity and phenotype of NK cells were evaluated by flow cytometry. It was found that activation of NK cells depended on IL-2 and was registered on day 2 of incubation with IL-2. In cultures with mitogen-activated lymphocytes, cytotoxicity was observed after 5-6 days. Cytotoxicity of NK correlated with significant decrease in CD16+ and increase in CD56+ NK and with reduction of mesenchymal stromal cell viability. Thus, the main mechanism of elimination of mesenchymal stromal cells is cytotoxicity of NK cells that depended on IL-2 production.
Databáze: MEDLINE
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