| Autor: |
Fukushi K; Kobe University Biosignal Research Center, 1-1, Rokkodai-cho, Nada-ku, Kobe, Hyogo, 657-8501, Japan. ppkp13580@leto.eonet.ne.jp., Fujita Y; Kobe University Faculty of Maritime Sciences, 5-1-1 Fukaeminami-machi, Higashinada-ku, Kobe, Hyogo, 658-0022, Japan., Nonogaki J; Kobe University Faculty of Maritime Sciences, 5-1-1 Fukaeminami-machi, Higashinada-ku, Kobe, Hyogo, 658-0022, Japan., Tsujimoto JI; Kiso Chemical Enterprises Ltd., 1 Maya-futo, Nada-ku, Kobe, Hyogo, 657-0854, Japan., Hattori T; Kobe University Graduate School of Maritime Sciences, 5-1-1 Fukae-minamimachi, Higashinada-ku, Kobe, Hyogo, 658-0022, Japan., Inui H; Kobe University Biosignal Research Center, 1-1, Rokkodai-cho, Nada-ku, Kobe, Hyogo, 657-8501, Japan. hinui@kobe-u.ac.jp., Beškoski VP; University of Belgrade Faculty of Chemistry, Studentski trg 12-16, Belgrade, 11000, Serbia., Hotta H; Kobe University Graduate School of Maritime Sciences, 5-1-1 Fukae-minamimachi, Higashinada-ku, Kobe, Hyogo, 658-0022, Japan., Hayashi M; Kobe University Graduate School of Maritime Sciences, 5-1-1 Fukae-minamimachi, Higashinada-ku, Kobe, Hyogo, 658-0022, Japan., Nakano T; Osaka University Research Center for Environmental Preservation, 2-4 Yamadaoka, Suita, Osaka, 565-0871, Japan. |
| Abstrakt: |
We developed capillary zone electrophoresis (CZE) with indirect UV detection for the determination of fluoride (F - ) in seawater using transient isotachophoresis (tITP) as an on-line concentration procedure. A method of correcting sample salinity effects was also proposed so that F - concentrations were obtained using a calibration graph. The proposed method is simple: it requires no sample pretreatment aside from dilution. The following optimum conditions were established: background electrolyte (BGE), 5 mM 2,6-pyridinedicarboxylic acid (PDC) adjusted to pH 3.5 containing 0.03% m/v hydroxypropyl methylcellulose (HPMC); detection wavelength, 200 nm; vacuum (50 kPa) injection period of sample, 5 s (254 nL); and applied voltage, 23 kV with the sample inlet side as the cathode. The limit of detection (LOD, S/N = 3) and limit of quantification (LOQ, S/N = 10) for F - reached 0.024 and 0.070 mg/L, respectively. The respective values of the relative standard deviation (RSD) of the peak area, peak height, and migration time for F - were 2.5, 3.4, and 0.30%. The proposed method was applied for the determination of F - in seawater samples collected from coastal waters of western Japan during August 26-28, 2014. Both results obtained using standard addition method and a calibration graph agreed with those obtained using a conventional spectrophotometric method. |
