Induction of oxidative stress by chlorothalonil in the estuarine polychaete Laeonereis acuta.

Autor: da Silva Barreto J; Instituto de Ciências Biológicas (ICB), Universidade Federal do Rio Grande - FURG, Rio Grande, RS, Brazil; Programa de Pós-Graduação em Ciências Fisiológicas - FURG, Brazil., de Melo Tarouco F; Instituto de Ciências Biológicas (ICB), Universidade Federal do Rio Grande - FURG, Rio Grande, RS, Brazil; Programa de Pós-Graduação em Ciências Fisiológicas - FURG, Brazil., de Godoi FGA; Instituto de Ciências Biológicas (ICB), Universidade Federal do Rio Grande - FURG, Rio Grande, RS, Brazil., Geihs MA; Instituto de Oceanografia (IO), Universidade Federal do Rio Grande - FURG, Rio Grande, RS, Brazil., Abreu FEL; Instituto de Oceanografia (IO), Universidade Federal do Rio Grande - FURG, Rio Grande, RS, Brazil; Programa de Pós-Graduação em Oceanografia Física, Química e Geológica - PPGOFQG - IO - FURG, Brazil., Fillmann G; Instituto de Oceanografia (IO), Universidade Federal do Rio Grande - FURG, Rio Grande, RS, Brazil; Programa de Pós-Graduação em Oceanografia Física, Química e Geológica - PPGOFQG - IO - FURG, Brazil., Sandrini JZ; Instituto de Ciências Biológicas (ICB), Universidade Federal do Rio Grande - FURG, Rio Grande, RS, Brazil; Programa de Pós-Graduação em Ciências Fisiológicas - FURG, Brazil., da Rosa CE; Instituto de Ciências Biológicas (ICB), Universidade Federal do Rio Grande - FURG, Rio Grande, RS, Brazil; Programa de Pós-Graduação em Ciências Fisiológicas - FURG, Brazil. Electronic address: carlosrosa@furg.br.
Jazyk: angličtina
Zdroj: Aquatic toxicology (Amsterdam, Netherlands) [Aquat Toxicol] 2018 Mar; Vol. 196, pp. 1-8. Date of Electronic Publication: 2017 Dec 15.
DOI: 10.1016/j.aquatox.2017.12.004
Abstrakt: Chlorothalonil is an active biocide applied in antifouling paints, and also used as fungicide in agricultural activities with the purpose to protect plants from foliar and seed diseases. Thus, the aim of this study was to evaluate the effects of chlorothalonil exposure on biochemical biomarkers of oxidative metabolism as well as on cholinesterases in the estuarine polychaete Laeonereis acuta. Animals were exposed for 24 and 96 h to the following nominal concentrations of chlorothalonil: 0.1, 10.0 and 100.0 μg/L. The antioxidant capacity against peroxyl radicals (ACAP) and the activity of the enzymes catalase (CAT), superoxide dismutase (SOD), glutathione S-transferase (GST), glutamate cysteine ligase (GCL), acetylcholinesterase (AChE) and propionylcholinesterase (PChE) were evaluated in whole-body tissue. In addition, the levels of reduced glutathione (GSH), lipid peroxidation (LPO), glycogen and lactate levels were also analyzed. A reduction in ACAP levels was observed in animals exposed to the higher chlorothalonil concentration, concomitantly with an induction of GST activity as well as diminution in GSH content in these animals. This disturbance in the redox state of animal tissues leads to an oxidative stress situation, resulting in an induction in LPO levels. It was also demonstrated that chlorothalonil exposure causes alteration in AChE activity, possibly related to damage to membrane lipids. These results demonstrated that chlorothalonil possesses harmful effects to estuarine animals and its use as antifouling biocide has to be carefully reconsidered in risk analysis studies.
(Copyright © 2017 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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