Dissection of affinity captured LINE-1 macromolecular complexes.

Autor:	Taylor MS; Department of Pathology, Massachusetts General Hospital, Boston, United States., Altukhov I; Moscow Institute of Physics and Technology, Dolgoprudny, Russia., Molloy KR; Laboratory of Mass Spectrometry and Gaseous Ion Chemistry, The Rockefeller University, New York, United States., Mita P; Department of Biochemistry and Molecular Pharmacology, Institute for Systems Genetics, NYU Langone Health, New York, United States., Jiang H; Laboratory of Cellular and Structural Biology, The Rockefeller University, New York, United States., Adney EM; Department of Biochemistry and Molecular Pharmacology, Institute for Systems Genetics, NYU Langone Health, New York, United States.; McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, United States., Wudzinska A; McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, United States., Badri S; Department of Pathology, NYU Langone Health, New York, United States., Ischenko D; Moscow Institute of Physics and Technology, Dolgoprudny, Russia., Eng G; Department of Pathology, Massachusetts General Hospital, Boston, United States., Burns KH; Laboratory of Cellular and Structural Biology, The Rockefeller University, New York, United States.; Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, United States., Fenyö D; Department of Biochemistry and Molecular Pharmacology, Institute for Systems Genetics, NYU Langone Health, New York, United States., Chait BT; Laboratory of Mass Spectrometry and Gaseous Ion Chemistry, The Rockefeller University, New York, United States., Alexeev D; Novosibirsk State University, Novosibirsk, Russia., Rout MP; Laboratory of Cellular and Structural Biology, The Rockefeller University, New York, United States., Boeke JD; Department of Biochemistry and Molecular Pharmacology, Institute for Systems Genetics, NYU Langone Health, New York, United States., LaCava J; Department of Biochemistry and Molecular Pharmacology, Institute for Systems Genetics, NYU Langone Health, New York, United States.; Laboratory of Cellular and Structural Biology, The Rockefeller University, New York, United States.
Jazyk:	angličtina
Zdroj:	ELife [Elife] 2018 Jan 08; Vol. 7. Date of Electronic Publication: 2018 Jan 08.
DOI:	10.7554/eLife.30094
Abstrakt:	Long Interspersed Nuclear Element-1 (LINE-1, L1) is a mobile genetic element active in human genomes. L1-encoded ORF1 and ORF2 proteins bind L1 RNAs, forming ribonucleoproteins (RNPs). These RNPs interact with diverse host proteins, some repressive and others required for the L1 lifecycle. Using differential affinity purifications, quantitative mass spectrometry, and next generation RNA sequencing, we have characterized the proteins and nucleic acids associated with distinctive, enzymatically active L1 macromolecular complexes. Among them, we describe a cytoplasmic intermediate that we hypothesize to be the canonical ORF1p/ORF2p/L1 - RNA-containing RNP, and we describe a nuclear population containing ORF2p, but lacking ORF1p, which likely contains host factors participating in target-primed reverse transcription. Competing Interests: MT, IA, KM, PM, HJ, EA, AW, SB, DI, GE, KB, DF, BC, DA, MR, JB, JL No competing interests declared (© 2018, Taylor et al.)
Databáze:	MEDLINE
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