| Autor: |
Trindade de Castro MC; a School of Biological and Marine Science , University of Plymouth , Plymouth , UK.; b Plymouth Marine Laboratory , Plymouth , UK.; c Directorate of Ports and Coasts , Rio de Janeiro , RJ , Brazil., Veldhuis MJW; d MES , WK , Den Burg , The Netherlands. |
| Jazyk: |
angličtina |
| Zdroj: |
Environmental technology [Environ Technol] 2019 Apr; Vol. 40 (11), pp. 1455-1466. Date of Electronic Publication: 2018 Jan 24. |
| DOI: |
10.1080/09593330.2017.1423117 |
| Abstrakt: |
In the Wadden Sea, the Netherlands, and at L4 in the English Channel, UK, the size class distribution of phytoplankton was investigated with respect to the size range >10-≤50 µm identified by the IMO Ballast Water Convention. Size fractionation using 10 µm mesh filtration showed considerable size bias; 23.1% of >10 µm cells were still present in the <10 µm, but 21.8% of the smaller size cells were also retained on the mesh, resulting in an overestimated number of cells/mL by as much as a factor of 5.4. Flowcytometry measurements indicated that the phytoplankton in the size range 2-50 µm was dominated by the smaller size (<10 µm) at both sites. For the >10-≤50 µm size, these were on average 3.6% and 2% in the Wadden Sea and at L4, respectively. In terms of chlorophyll biomass, they represented 28.7% and 12%, respectively. The filtration method resulted in much higher chlorophyll values for 10-50 µm size range: 53.7% in the Wadden Sea and 38% at L4. This overestimation appears to be caused by cells in 6-10 µm size range being retained on the mesh. These findings are relevant in the context of the size class distribution based on flowcytometry and semi-quantification using chlorophyll as proxy for cell density. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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