Sequence and functional divergence of gametocyte-specific parasitophorous vacuole membrane proteins in Plasmodium parasites.

Autor: Deligianni E; Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology-Hellas, Heraklion, Greece., Andreadaki M; Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology-Hellas, Heraklion, Greece., Koutsouris K; Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology-Hellas, Heraklion, Greece; Department of Biology, University of Crete, Heraklion, Greece., Siden-Kiamos I; Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology-Hellas, Heraklion, Greece. Electronic address: inga@imbb.forth.gr.
Jazyk: angličtina
Zdroj: Molecular and biochemical parasitology [Mol Biochem Parasitol] 2018 Mar; Vol. 220, pp. 15-18. Date of Electronic Publication: 2018 Jan 04.
DOI: 10.1016/j.molbiopara.2018.01.002
Abstrakt: Plasmodium parasites develop within red blood cells in a Parasitophorous Vacuole enclosed by a Membrane, the PVM. The protein family ETRAMP (Early Transcribed Membrane Protein) comprises small proteins inserted in the PVM via a single transmembrane domain. Among those, Pfs16 is specifically found in P. falciparum gametocyte PVM. The P. berghei gene PBANKA_1003900 is syntenic with pfs16. The encoded proteins have a similar domain structure but the overall protein similarity is low. A transcript of the P. berghei gene is only found in gametocytes and ookinetes and a C-terminal mCherry fusion of the protein revealed its presence only in gametocytes. A knock-out mutant of the PBANKA_1003900 gene was not affected in sexual development and ookinete formation was similar to WT. The mutation had no adverse effect on transmission through the mosquito although there was a reduction of the number of oocysts formed by the mutant parasites.
(Copyright © 2018 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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