The ellagitannin HeT induces electrolyte leakage, calcium influx and the accumulation of nitric oxide and hydrogen peroxide in strawberry.

Autor: Martos GG; Instituto Superior de Investigaciones Biológicas (INSIBIO), CONICET-UNT, and Instituto de Química Biológica 'Dr. Bernabé Bloj', Facultad de Bioquímica, Química y Farmacia, UNT, Chacabuco 461, T4000ILI, San Miguel de Tucumán, Argentina., Mamaní A; Instituto Superior de Investigaciones Biológicas (INSIBIO), CONICET-UNT, and Instituto de Química Biológica 'Dr. Bernabé Bloj', Facultad de Bioquímica, Química y Farmacia, UNT, Chacabuco 461, T4000ILI, San Miguel de Tucumán, Argentina., Filippone MP; Sección Biotecnología de la Estación Experimental Agroindustrial Obispo Colombres (EEAOC)-Unidad Asociada al INSIBIO, Av. William Cross 3150, Las Talitas, 4101, Tucumán, Argentina., Castagnaro AP; Sección Biotecnología de la Estación Experimental Agroindustrial Obispo Colombres (EEAOC)-Unidad Asociada al INSIBIO, Av. William Cross 3150, Las Talitas, 4101, Tucumán, Argentina., Díaz Ricci JC; Instituto Superior de Investigaciones Biológicas (INSIBIO), CONICET-UNT, and Instituto de Química Biológica 'Dr. Bernabé Bloj', Facultad de Bioquímica, Química y Farmacia, UNT, Chacabuco 461, T4000ILI, San Miguel de Tucumán, Argentina. Electronic address: juan@fbqf.unt.edu.ar.
Jazyk: angličtina
Zdroj: Plant physiology and biochemistry : PPB [Plant Physiol Biochem] 2018 Feb; Vol. 123, pp. 400-405. Date of Electronic Publication: 2017 Dec 27.
DOI: 10.1016/j.plaphy.2017.12.035
Abstrakt: HeT (1-0-galloyl-2,3; 4,6-bis-hexahydroxydiphenoyl-β-D-glucopyranose) is a penta-esterified ellagitannin obtained from strawberry leaves. Previous studies have shown that foliar application of HeT prior to inoculation with a virulent pathogen increases the resistance toward Colletotrichum acutatum in strawberry plants and to Xanthomonas citri subsp. citri in lemon plants. In this work we report that HeT induces an immediate leak of electrolytes, the hyperpolarization of the cellular membrane, a rapid Ca 2+ influx to the cytoplasm during the first few seconds, which in turn modulates the accumulation of nitric oxide 5 min after treatment. At longer times, a biphasic accumulation of H 2 O 2 with peaks at 2 and 5 h post treatment could be observed. In addition, HeT elicited the increase of alternative oxidase capacity during the first 12 h post treatment.
(Copyright © 2017 Elsevier Masson SAS. All rights reserved.)
Databáze: MEDLINE
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