Evaluation of the antioxidant and gastroprotective activity and HPLC analysis of the hydroalcoholic extract of Tocoyena formosa leaves (Cham. & Schlecht) K. Schum.

Autor: Alves Santana Cesário FR; Department of Biological Chemistry, Laboratory of Pharmacology and Molecular Chemistry Regional University of Cariri, Regional University of Cariri, Crato, CE, 63105-000, Brazil., Rodrigues de Albuquerque T; Department of Biological Chemistry, Laboratory of Pharmacology and Molecular Chemistry Regional University of Cariri, Regional University of Cariri, Crato, CE, 63105-000, Brazil., Fernandes da Silva BA; Department of Biological Chemistry, Laboratory of Pharmacology and Molecular Chemistry Regional University of Cariri, Regional University of Cariri, Crato, CE, 63105-000, Brazil., Mendes de Lacerda G; Department of Biological Chemistry, Laboratory of Pharmacology and Molecular Chemistry Regional University of Cariri, Regional University of Cariri, Crato, CE, 63105-000, Brazil., Rodrigues LB; Department of Biological Chemistry, Laboratory of Pharmacology and Molecular Chemistry Regional University of Cariri, Regional University of Cariri, Crato, CE, 63105-000, Brazil., Brito Pereira Bezerra Martins AO; Department of Biological Chemistry, Laboratory of Pharmacology and Molecular Chemistry Regional University of Cariri, Regional University of Cariri, Crato, CE, 63105-000, Brazil., Quintans-Júnior LJ; Department of Physiology, Federal University of Sergipe, Avenue Marechal Rondon, S/N - Rosa Elze, São Cristóvão, SE, 49100-000, Brazil., Guedes da Silva Almeida JR; Natural Resources of the Semi-Arid Program, Federal University of the Vale São Francisco, Av. José de Sá Maniçoba, s/n, Campus Universitário, Petrolina, PE, Brazil., Vale ML; Department of Pharmacology, Federal University of Ceara, Avenue Av. da Universidade, 2853 - Benfica, Fortaleza, CE, 60020-180, Brazil., Melo Coutinho HD; Department of Biological Chemistry, Laboratory of Microbiology and Biology Molecular Regional University of Cariri, Regional University of Cariri, Crato, CE, 63105-000, Brazil. Electronic address: hdmcoutinho@gmail.com., Alencar de Menezes IR; Department of Biological Chemistry, Laboratory of Pharmacology and Molecular Chemistry Regional University of Cariri, Regional University of Cariri, Crato, CE, 63105-000, Brazil.
Jazyk: angličtina
Zdroj: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association [Food Chem Toxicol] 2018 Feb; Vol. 112, pp. 355-362. Date of Electronic Publication: 2017 Dec 30.
DOI: 10.1016/j.fct.2017.12.058
Abstrakt: The peptic ulcer is a gastric disorder that affects millions of people and yet they cause many side effects. In this sense, natural products represent an important alternative to the discovery of compounds with gastroprotective activity. The present work has as its objective to evaluate the antioxidant and gastroprotective activity of the Hydroalcoholic Extract of Leaves from Tocoyena formosa (Cham. & Schlecht.) K. Schum (HELTF), this being much emphasized in traditional medicine for inflammatory morbidities and gastric symptoms. For the evaluation of the antioxidant activity, FRAP and DPPH tests were carried out, and for the evaluation of the gastroprotective activity, gastric lesion induction by ethanol, acidified ethanol, indomethacin and physical barrier tests were used. Antioxidant assay of HELTF revealed an EC50 of 558.66 μM FeSO4/g and IC50 of 189.78 μg/ml for FRAP and DPPH respectively. In gastroprotective, in ethanol model, all the doses presented significant activity in comparison to controls, however, in the gastric lesion induction test by acidified ethanol and indomethacin, only the dose of 200 mg/kg presented with significance. In the physical barrier test presented evidence that protection by the formation of a protective layer associated with mucus. Concluded, therefore, that HELTF possesses antioxidant and significate gastroprotective activity.
(Copyright © 2018 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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