| Autor: |
Lalaouna D; Department of Biochemistry, RNA Group, Université de Sherbrooke, Sherbrooke, Québec, Canada., Prévost K; Department of Biochemistry, RNA Group, Université de Sherbrooke, Sherbrooke, Québec, Canada., Laliberté G; Department of Biochemistry, RNA Group, Université de Sherbrooke, Sherbrooke, Québec, Canada., Houé V; Department of Biochemistry, RNA Group, Université de Sherbrooke, Sherbrooke, Québec, Canada., Massé E; Department of Biochemistry, RNA Group, Université de Sherbrooke, Sherbrooke, Québec, Canada. |
| Abstrakt: |
Small RNAs are key components of complex regulatory networks. These molecules can integrate multiple cellular signals to control specific target mRNAs. The recent development of high-throughput methods tremendously helped to characterize the full targetome of sRNAs. Using MS2-affinity purification coupled with RNA sequencing (MAPS) technology, we reveal the targetomes of two sRNAs, CyaR and RprA. Interestingly, both CyaR and RprA interact with the 5'-UTR of hdeD mRNA, which encodes an acid-resistance membrane protein. We demonstrate that CyaR classically binds to the RBS of hdeD, interfering with translational initiation. We identified an A/U-rich motif on hdeD, which is bound by the RNA chaperone Hfq. Our results indicate that binding of this motif by Hfq is required for CyaR-induced degradation of hdeD mRNA. Additional data suggest that two molecules of RprA must bind the 5'-UTR of hdeD to block translation initiation. Surprisingly, while both CyaR and RprA sRNAs bind to the same motif on hdeD mRNA, RprA solely acts at the translational level, leaving the target RNA intact. By interchanging the seed region of CyaR and RprA sRNAs, we also swap their regulatory behavior. These results suggest that slight changes in the seed region could modulate the regulation of target mRNAs. |
