A combination of NMR and liquid chromatography to characterize the protective effects of Rhus tripartita extracts on ethanol-induced toxicity and inflammation on intestinal cells.

Autor: Ben Barka Z; Laboratory of Integrated Physiology, Faculty of Science of Bizerte, University of Carthage Tunisia, 7021 Jarzouna, Bizerte, Tunisia; Laboratory of Cellular, Nutritional and Toxicological Biochemistry (BCNT), Life Sciences Institute, Université catholique de Louvain (UCL), BE1348 Louvain-la-Neuve, Belgium., Grintzalis K; Laboratory of Cellular, Nutritional and Toxicological Biochemistry (BCNT), Life Sciences Institute, Université catholique de Louvain (UCL), BE1348 Louvain-la-Neuve, Belgium., Polet M; Laboratory of Cellular, Nutritional and Toxicological Biochemistry (BCNT), Life Sciences Institute, Université catholique de Louvain (UCL), BE1348 Louvain-la-Neuve, Belgium., Heude C; NERC Biomolecular Analysis Facility-Metabolomics Node (NBAF-B), School of Biosciences, University of Birmingham, Birmingham, B15 2TT, United Kingdom., Sommer U; NERC Biomolecular Analysis Facility-Metabolomics Node (NBAF-B), School of Biosciences, University of Birmingham, Birmingham, B15 2TT, United Kingdom., Ben Miled H; Laboratory of Integrated Physiology, Faculty of Science of Bizerte, University of Carthage Tunisia, 7021 Jarzouna, Bizerte, Tunisia., Ben Rhouma K; Laboratory of Integrated Physiology, Faculty of Science of Bizerte, University of Carthage Tunisia, 7021 Jarzouna, Bizerte, Tunisia., Mohsen S; Laboratory of Integrated Physiology, Faculty of Science of Bizerte, University of Carthage Tunisia, 7021 Jarzouna, Bizerte, Tunisia., Tebourbi O; Laboratory of Integrated Physiology, Faculty of Science of Bizerte, University of Carthage Tunisia, 7021 Jarzouna, Bizerte, Tunisia., Schneider YJ; Laboratory of Cellular, Nutritional and Toxicological Biochemistry (BCNT), Life Sciences Institute, Université catholique de Louvain (UCL), BE1348 Louvain-la-Neuve, Belgium. Electronic address: yjs@uclouvain.be.
Jazyk: angličtina
Zdroj: Journal of pharmaceutical and biomedical analysis [J Pharm Biomed Anal] 2018 Feb 20; Vol. 150, pp. 347-354. Date of Electronic Publication: 2017 Dec 19.
DOI: 10.1016/j.jpba.2017.12.032
Abstrakt: Consumption of ethanol may have severe effects on human organs and tissues and lead to acute and chronic inflammation of internal organs. The present study aims at investigating the potential protective effects of three different extracts prepared from the leaves, root, and stem of the sumac, Rhus tripartita, against ethanol-induced toxicity and inflammation using intestinal cells as a cell culture system, in vitro model of the intestinal mucosa. The results showed an induction of cytotoxicity by ethanol, which was partially reversed by co-administration of the plant extracts. As part of investigating the cellular response and the mechanism of toxicity, the role of reduced thiols and glutathione-S-transferases were assessed. In addition, intestinal cells were artificially imposed to an inflammation state and the anti-inflammatory effect of the extracts was estimated by determination of interleukin-8. Finally, a detailed characterization of the contents of the three plant extracts by high resolution Nuclear Magnetic Resonance (NMR) spectroscopy and mass spectrometry revealed significant differences in their chemical compositions.
(Copyright © 2017 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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