| Autor: |
Gómez-Villafuertes R; Biochemistry and Molecular Biology Department, Faculty of Veterinary medicine, Complutense University; University Institute for Neurochemistry Research (IUIN); Instituto de Investigación Sanitaria del Hospital Clínico San Carlos (IdISSC)., Paniagua-Herranz L; Biochemistry and Molecular Biology Department, Faculty of Veterinary medicine, Complutense University; University Institute for Neurochemistry Research (IUIN); Instituto de Investigación Sanitaria del Hospital Clínico San Carlos (IdISSC)., Gascon S; Institute of Stem Cell Research, Helmholtz Center Munich, Neuherberg/Munich, Germany Physiological Genomics, Biomedical Center, Ludwig-Maximilians University Munich; Toxicology and Pharmacology Department, Faculty of Veterinary medicine, Complutense University., de Agustín-Durán D; Biochemistry and Molecular Biology Department, Faculty of Veterinary medicine, Complutense University; University Institute for Neurochemistry Research (IUIN); Instituto de Investigación Sanitaria del Hospital Clínico San Carlos (IdISSC)., Ferreras MO; Biochemistry and Molecular Biology Department, Faculty of Veterinary medicine, Complutense University; University Institute for Neurochemistry Research (IUIN); Instituto de Investigación Sanitaria del Hospital Clínico San Carlos (IdISSC)., Gil-Redondo JC; Biochemistry and Molecular Biology Department, Faculty of Veterinary medicine, Complutense University; University Institute for Neurochemistry Research (IUIN); Instituto de Investigación Sanitaria del Hospital Clínico San Carlos (IdISSC)., Queipo MJ; Biochemistry and Molecular Biology Department, Faculty of Veterinary medicine, Complutense University; University Institute for Neurochemistry Research (IUIN); Instituto de Investigación Sanitaria del Hospital Clínico San Carlos (IdISSC)., Menendez-Mendez A; Biochemistry and Molecular Biology Department, Faculty of Veterinary medicine, Complutense University; University Institute for Neurochemistry Research (IUIN); Instituto de Investigación Sanitaria del Hospital Clínico San Carlos (IdISSC)., Pérez-Sen R; Biochemistry and Molecular Biology Department, Faculty of Veterinary medicine, Complutense University; University Institute for Neurochemistry Research (IUIN); Instituto de Investigación Sanitaria del Hospital Clínico San Carlos (IdISSC)., Delicado EG; Biochemistry and Molecular Biology Department, Faculty of Veterinary medicine, Complutense University; University Institute for Neurochemistry Research (IUIN); Instituto de Investigación Sanitaria del Hospital Clínico San Carlos (IdISSC)., Gualix J; Biochemistry and Molecular Biology Department, Faculty of Veterinary medicine, Complutense University; University Institute for Neurochemistry Research (IUIN); Instituto de Investigación Sanitaria del Hospital Clínico San Carlos (IdISSC)., Costa MR; Brain Institute, Federal University of Rio Grande do Norte., Schroeder T; Department of Biosystems Science and Engineering, Eidgenössische Technische Hochschule (ETH) Zurich., Miras-Portugal MT; Biochemistry and Molecular Biology Department, Faculty of Veterinary medicine, Complutense University; University Institute for Neurochemistry Research (IUIN); Instituto de Investigación Sanitaria del Hospital Clínico San Carlos (IdISSC)., Ortega F; Biochemistry and Molecular Biology Department, Faculty of Veterinary medicine, Complutense University; University Institute for Neurochemistry Research (IUIN); Instituto de Investigación Sanitaria del Hospital Clínico San Carlos (IdISSC); fortegao@ucm.es. |
| Abstrakt: |
Understanding the mechanisms that control critical biological events of neural cell populations, such as proliferation, differentiation, or cell fate decisions, will be crucial to design therapeutic strategies for many diseases affecting the nervous system. Current methods to track cell populations rely on their final outcomes in still images and they generally fail to provide sufficient temporal resolution to identify behavioral features in single cells. Moreover, variations in cell death, behavioral heterogeneity within a cell population, dilution, spreading, or the low efficiency of the markers used to analyze cells are all important handicaps that will lead to incomplete or incorrect read-outs of the results. Conversely, performing live imaging and single cell tracking under appropriate conditions represents a powerful tool to monitor each of these events. Here, a time-lapse video-microscopy protocol, followed by post-processing, is described to track neural populations with single cell resolution, employing specific software. The methods described enable researchers to address essential questions regarding the cell biology and lineage progression of distinct neural populations. |
