Antigen Analysis of Pre-Eclamptic Plasma Antibodies Using Escherichia Coli Proteome Chips.

Autor:	Hsu TY; From the ‡Department of Obstetrics and Gynecology, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, Taiwan; cchen103@gmail.com tyhsu@cgmh.org.tw., Lin JM; §Graduate Institute of Systems Biology and Bioinformatics, National Central University, Jhongli 32001, Taiwan.; ¶Department of Biomedical Science and Engineering, National Central University, Jhongli 32001, Taiwan., Nguyen MT; §Graduate Institute of Systems Biology and Bioinformatics, National Central University, Jhongli 32001, Taiwan.; ¶Department of Biomedical Science and Engineering, National Central University, Jhongli 32001, Taiwan., Chung FH; §Graduate Institute of Systems Biology and Bioinformatics, National Central University, Jhongli 32001, Taiwan.; ¶Department of Biomedical Science and Engineering, National Central University, Jhongli 32001, Taiwan., Tsai CC; From the ‡Department of Obstetrics and Gynecology, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, Taiwan., Cheng HH; From the ‡Department of Obstetrics and Gynecology, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, Taiwan., Lai YJ; From the ‡Department of Obstetrics and Gynecology, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, Taiwan., Hung HN; From the ‡Department of Obstetrics and Gynecology, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, Taiwan., Chen CS; §Graduate Institute of Systems Biology and Bioinformatics, National Central University, Jhongli 32001, Taiwan; cchen103@gmail.com tyhsu@cgmh.org.tw.; ¶Department of Biomedical Science and Engineering, National Central University, Jhongli 32001, Taiwan.; ‖Department of Food Safety/Hygiene and Risk Management, College of Medicine, National Cheng Kung University, Tainan City 704, Taiwan.
Jazyk:	angličtina
Zdroj:	Molecular & cellular proteomics : MCP [Mol Cell Proteomics] 2018 Aug; Vol. 17 (8), pp. 1457-1469. Date of Electronic Publication: 2017 Dec 28.
DOI:	10.1074/mcp.RA117.000139
Abstrakt:	Pre-eclampsia is one of the main causes of perinatal mortality and morbidity. Many biomarkers for diagnosing pre-eclampsia have been found but most have low accuracy. Therefore, a potential marker that can detect pre-eclampsia with high accuracy is required. Infection has been reported as a cause of pre-eclampsia. In recent years, protein microarray chips have been recognized as a strong and robust tool for profiling antibodies for infection diagnoses. The purpose of the present study was to profile antibodies in the human plasma of healthy and pre-eclamptic pregnancies to identify suitable biomarkers. In this study, an Escherichia coli chip was probed with samples from 29 individuals (16 pre-eclamptic women and 13 healthy pregnant women) to profile plasma antibodies. Bioinformatics tools were used to analyze the results, discover conserved motifs, compare against the entire human proteome, and perform protein functional analysis. An antibody classifier was identified using k -top scoring pairs and additional samples for a blinded test were collected. The findings indicated that compared with the healthy women, the pre-eclamptic women exhibited 108 and 130 differentially immunogenic proteins against human immunoglobulins G and M, respectively. In addition, pre-eclamptic women developed more immunoglobulin G but less immunoglobulin M against bacterial surface proteins compared with healthy women. The k-top scoring pairs identified five pairs of immunogenic proteins as classifiers with a high accuracy of 90% in the blind test. [AG] [ISV] GV [AE] L [LF] and [IV] [IV] RI [AG] [AD] E were the consensus motifs observed in immunogenic proteins in the immunoglobulin G and immunoglobulin M of pre-eclamptic women, respectively, whereas GA [AG] [AL] L [LF] and [SRY] [IQML] [ILV] [ILV] [ACG] GI [GH] [AEF] [AK] [ATY] [RG] N [IV] were observed in the immunoglobulins G and immunoglobulin M of healthy women, respectively. (© 2018 by The American Society for Biochemistry and Molecular Biology, Inc.)
Databáze:	MEDLINE
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