Preclinical efficacy and immunogenicity assessment to show that a chimeric Plasmodium falciparum UB05-09 antigen could be a malaria vaccine candidate.

Autor: Dinga JN; Biotechnology Unit, Faculty of Science, University of Buea, Buea, Cameroon.; Department of Biochemistry and Molecular Biology, Faculty of Science, University of Buea, Buea, Cameroon., Gamua SD; Department of Biochemistry and Molecular Biology, Faculty of Science, University of Buea, Buea, Cameroon., Ghogomu SM; Department of Biochemistry and Molecular Biology, Faculty of Science, University of Buea, Buea, Cameroon., Titanji VPK; Biotechnology Unit, Faculty of Science, University of Buea, Buea, Cameroon.; Faculty of Science, Engineering and Technology, Cameroon Christian University Institute, Bali, Cameroon.
Jazyk: angličtina
Zdroj: Parasite immunology [Parasite Immunol] 2018 Mar; Vol. 40 (3). Date of Electronic Publication: 2018 Jan 25.
DOI: 10.1111/pim.12514
Abstrakt: Although it is generally agreed that an effective vaccine would greatly accelerate the control of malaria, the lone registered malaria vaccine Mosquirix™ has an efficacy of 30%-60% that wanes rapidly, indicating a need for improved second-generation malaria vaccines. Previous studies suggested that immune responses to a chimeric Plasmodium falciparum antigen UB05-09 are associated with immune protection against malaria. Herein, the preclinical efficacy and immunogenicity of UB05-09 are tested. Growth inhibition assay was employed to measure the effect of anti-UB05-09 antibodies on P. falciparum growth in vitro. BALB/c mice were immunized with UB05-09 and challenged with the lethal Plasmodium yoelii 17XL infection. ELISA was used to measure antigen-specific antibody production. ELISPOT assays were employed to measure interferon-gamma production ex vivo after stimulation with chimeric UB05-09 and its constituent antigens. Purified immunoglobulins raised in rabbits against UB05-09 significantly inhibited P. falciparum growth in vitro compared to that of its respective constituent antigens. A combination of antibodies to UB05-09 and the apical membrane antigen (AMA1) completely inhibited P. falciparum growth in culture. Immunization of BALB/c mice with recombinant UB05-09 blocked parasitaemia and protected them against lethal P. yoelii 17XL challenge infection. These data suggest that UB05-09 is a malaria vaccine candidate that could be developed further and used in conjunction with AMA1 to create a potent malaria vaccine.
(© 2017 The Authors. Parasite Immunology Published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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