Identification and expression of a unique neonatal variant of the GABA A receptor α 3 subunit.

Autor: Miller SM; The University of Queensland, Perinatal Research Centre, UQ Centre for Clinical Research, Royal Brisbane and Women's Hospital, Brisbane, QLD, 4029, Australia., Pelly S; The University of Queensland, Perinatal Research Centre, UQ Centre for Clinical Research, Royal Brisbane and Women's Hospital, Brisbane, QLD, 4029, Australia., Kalanjati VP; The University of Queensland, Perinatal Research Centre, UQ Centre for Clinical Research, Royal Brisbane and Women's Hospital, Brisbane, QLD, 4029, Australia.; The Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia., Lee A; The University of Queensland, Perinatal Research Centre, UQ Centre for Clinical Research, Royal Brisbane and Women's Hospital, Brisbane, QLD, 4029, Australia., Colditz PB; The University of Queensland, Perinatal Research Centre, UQ Centre for Clinical Research, Royal Brisbane and Women's Hospital, Brisbane, QLD, 4029, Australia., Bjorkman ST; The University of Queensland, Perinatal Research Centre, UQ Centre for Clinical Research, Royal Brisbane and Women's Hospital, Brisbane, QLD, 4029, Australia. t.bjorkman@uq.edu.au.
Jazyk: angličtina
Zdroj: Brain structure & function [Brain Struct Funct] 2018 Mar; Vol. 223 (2), pp. 1025-1033. Date of Electronic Publication: 2017 Dec 27.
DOI: 10.1007/s00429-017-1597-6
Abstrakt: The GABA A receptor provides the majority of inhibitory neurotransmission in the adult central nervous system but in immature brain is responsible for much of the excitatory drive, a requirement for normal brain development. It is well established that GABA A receptor subunit expression changes across the course of brain development. In the present study, we have identified a splice variant of the GABA A receptor α 3 subunit which appears unique to the developing brain, referred to here as the GABA A receptor α 3 subunit neonatal variant (GABA A receptor α 3N ). RT-PCR and sequence analysis revealed splicing of exon 8 of the α 3 subunit. Western blot analysis showed expression of GABA A receptor α 3N in the cortex of several neonatal species and significantly reduced expression of this splice variant in the corresponding adult brains. Expression was evident in multiple brain regions and decreased across development in the pig. Fractionation revealed differential cellular localisation in the parietal cortex, hippocampus and thalamus of the full-length GABA A receptor α 3 and GABA A receptor α 3N . Immunoprecipitation showed direct interaction with the GABA A receptor subunits α 1 and γ 2 but not with gephyrin.
Databáze: MEDLINE
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