African and Asian Zika Virus Isolates Display Phenotypic Differences Both In Vitro and In Vivo.

Autor:	Smith DR; Virology Division, United States Army Medical Research Institute of Infectious Diseases, Frederick, Maryland., Sprague TR; Virology Division, United States Army Medical Research Institute of Infectious Diseases, Frederick, Maryland., Hollidge BS; Virology Division, United States Army Medical Research Institute of Infectious Diseases, Frederick, Maryland., Valdez SM; Virology Division, United States Army Medical Research Institute of Infectious Diseases, Frederick, Maryland., Padilla SL; Virology Division, United States Army Medical Research Institute of Infectious Diseases, Frederick, Maryland., Bellanca SA; Virology Division, United States Army Medical Research Institute of Infectious Diseases, Frederick, Maryland., Golden JW; Virology Division, United States Army Medical Research Institute of Infectious Diseases, Frederick, Maryland., Coyne SR; Diagnostics Systems Division, United States Army Medical Research Institute of Infectious Diseases, Frederick, Maryland., Kulesh DA; Diagnostics Systems Division, United States Army Medical Research Institute of Infectious Diseases, Frederick, Maryland., Miller LJ; Virology Division, United States Army Medical Research Institute of Infectious Diseases, Frederick, Maryland., Haddow AD; Virology Division, United States Army Medical Research Institute of Infectious Diseases, Frederick, Maryland., Koehler JW; Diagnostics Systems Division, United States Army Medical Research Institute of Infectious Diseases, Frederick, Maryland., Gromowski GD; Walter Reed Army Institute of Research, Silver Spring, Maryland., Jarman RG; Walter Reed Army Institute of Research, Silver Spring, Maryland., Alera MTP; Department of Virology, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand., Yoon IK; International Vaccine Institute, Seoul, Republic of Korea., Buathong R; Department of Disease Control, Bureau of Epidemiology, Ministry of Public Health, Nonthaburi, Thailand., Lowen RG; Virology Division, United States Army Medical Research Institute of Infectious Diseases, Frederick, Maryland., Kane CD; Molecular and Translational Sciences Division, United States Army Medical Research Institute of Infectious Diseases, Frederick, Maryland., Minogue TD; Diagnostics Systems Division, United States Army Medical Research Institute of Infectious Diseases, Frederick, Maryland., Bavari S; Virology Division, United States Army Medical Research Institute of Infectious Diseases, Frederick, Maryland., Tesh RB; Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas.; Department of Pathology, University of Texas Medical Branch, Galveston, Texas.; Institute for Human Infections and Immunity, Center for Tropical Diseases, University of Texas Medical Branch, Galveston, Texas., Weaver SC; Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas.; Department of Pathology, University of Texas Medical Branch, Galveston, Texas.; Institute for Human Infections and Immunity, Center for Tropical Diseases, University of Texas Medical Branch, Galveston, Texas., Linthicum KJ; Center for Medical, Agricultural and Veterinary Entomology, Agricultural Research Service, United States Department of Agriculture, Gainesville, Florida., Pitt ML; Virology Division, United States Army Medical Research Institute of Infectious Diseases, Frederick, Maryland., Nasar F; Virology Division, United States Army Medical Research Institute of Infectious Diseases, Frederick, Maryland.; Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas.
Jazyk:	angličtina
Zdroj:	The American journal of tropical medicine and hygiene [Am J Trop Med Hyg] 2018 Feb; Vol. 98 (2), pp. 432-444. Date of Electronic Publication: 2017 Dec 21.
DOI:	10.4269/ajtmh.17-0685
Abstrakt:	Zika virus (ZIKV) is a mosquito-borne member of the genus Flavivirus that has emerged since 2007 to cause outbreaks in Africa, Asia, Oceania, and most recently, in the Americas. Here, we used an isolate history as well as genetic and phylogenetic analyses to characterize three low-passage isolates representing African (ArD 41525) and Asian (CPC-0740, SV0127-14) lineages to investigate the potential phenotypic differences in vitro and in vivo. The African isolate displayed a large plaque phenotype (∼3-4 mm) on Vero and HEK-293 cells, whereas the Asian isolates either exhibited a small plaque phenotype (∼1-2 mm) or did not produce any plaques. In multistep replication kinetics in nine different vertebrate and insect cell lines, the African isolate consistently displayed faster replication kinetics and yielded ∼10- to 10,000-fold higher peak virus titers (infectious or RNA copies) compared with the Asian isolates. Oral exposure of Aedes aegypti mosquitoes with the African isolate yielded higher infection and dissemination rates compared with the Asian isolates. Infection of Ifnar1 -/- mice with the African isolate produced a uniformly fatal disease, whereas infection with the Asian isolates produced either a delay in time-to-death or a significantly lower mortality rate. Last, the African isolate was > 10,000-fold more virulent than the Asian isolates in an interferon type I antibody blockade mouse model. These data demonstrate substantial phenotypic differences between low-passage African and Asian isolates both in vitro and in vivo and warrant further investigation. They also highlight the need for basic characterization of ZIKV isolates, as the utilization of the uncharacterized isolates could have consequences for animal model and therapeutic/vaccine development.
Databáze:	MEDLINE
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