Maternal undernutrition during early pregnancy inhibits postnatal growth of the tibia in the female offspring of rats by alteration of chondrogenesis.

Autor: Kimura T; Department of Anatomy, Shiga University of Medical Science, Shiga 520-2192, Japan., Hino K; Department of Anatomy, Shiga University of Medical Science, Shiga 520-2192, Japan., Kono T; Department of Anatomy, Shiga University of Medical Science, Shiga 520-2192, Japan., Takano A; Department of Medical Oncology, Shiga University of Medical Science, Shiga 520-2192, Japan., Nitta N; Department of Radiology, Shiga University of Medical Science, Shiga 520-2192, Japan., Ushio N; Department of Radiology, Shiga University of Medical Science, Shiga 520-2192, Japan., Hino S; Department of Medical Cell Biology, Institute of Molecular Embryology and Genetics, Kumamoto University, Kumamoto 860-8555, Japan., Takase R; Department of Medical Cell Biology, Institute of Molecular Embryology and Genetics, Kumamoto University, Kumamoto 860-8555, Japan., Kudo M; Department of Anatomy, Shiga University of Medical Science, Shiga 520-2192, Japan., Daigo Y; Department of Medical Oncology, Shiga University of Medical Science, Shiga 520-2192, Japan., Morita W; Department of Oral Functional Anatomy, Faculty of Dental Medicine, Hokkaido University, Hokkaido 060-8586, Japan., Nakao M; Department of Medical Cell Biology, Institute of Molecular Embryology and Genetics, Kumamoto University, Kumamoto 860-8555, Japan., Nakatsukasa M; Laboratory of Physical Anthropology, Kyoto University Graduate School of Science, Kyoto 606-8502, Japan., Tamagawa T; Department of Anatomy, Shiga University of Medical Science, Shiga 520-2192, Japan., Rafiq AM; Department of Anatomy, Faculty of Medicine, Shimane University, Shimane 693-8501, Japan., Matsumoto A; Department of Anatomy, Faculty of Medicine, Shimane University, Shimane 693-8501, Japan., Otani H; Department of Anatomy, Faculty of Medicine, Shimane University, Shimane 693-8501, Japan., Udagawa J; Department of Anatomy, Shiga University of Medical Science, Shiga 520-2192, Japan. Electronic address: udagawa@belle.shiga-med.ac.jp.
Jazyk: angličtina
Zdroj: General and comparative endocrinology [Gen Comp Endocrinol] 2018 May 01; Vol. 260, pp. 58-66. Date of Electronic Publication: 2017 Dec 23.
DOI: 10.1016/j.ygcen.2017.12.008
Abstrakt: Epidemiological research has suggested that birth weights are correlated with adult leg lengths. However, the relationship between prenatal undernutrition (UN) and postnatal leg growth remains controversial. We investigated the effects of UN during early pregnancy on postnatal hindlimb growth and determined whether early embryonic malnutrition affects the functions of postnatal chondrocytes in rats. Undernourished Wistar dams were fed 40% of the daily intake of rats in the control groups from gestational days 5.5-11.5, and femurs, tibias, and trunks or spinal columns were morphologically measured at birth and at 16 weeks of age in control and undernourished offspring of both sexes. We evaluated cell proliferation and differentiation of cultured chondrocytes derived from neonatal tibias of female offspring and determined chondrocyte-related gene expression levels in neonatal epiphysis and embryonic limb buds. Tibial lengths of undernourished female, but not male, offspring were longer at birth and shorter at 16 weeks of age (p < .05) compared with those of control rats. In chondrocyte culture studies, stimulating effects of IGF-1 on cell proliferation (p < .01) were significantly decreased and levels of type II collagen were lower in female undernourished offspring (p < .05). These phenomena were accompanied by decreased expression levels of Col2a1 and Igf1r and increased expression levels of Fgfr3 (p < .05), which might be attributable to the decreased expression of specificity protein 1 (p < .05), a key transactivator of Col2a1 and Igf1r. In conclusion, UN stress during early pregnancy reduces postnatal tibial growth in female offspring by altering the function of chondrocytes, likely reflecting altered expression of gene transactivators.
(Copyright © 2017 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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