Surface coating affects uptake of silver nanoparticles in neural stem cells.

Autor: Pongrac IM; University of Zagreb School of Medicine, Croatian Institute for Brain Research, Šalata 12, 10000, Zagreb, Croatia., Ahmed LB; University of Zagreb School of Medicine, Croatian Institute for Brain Research, Šalata 12, 10000, Zagreb, Croatia., Mlinarić H; University of Zagreb School of Medicine, Croatian Institute for Brain Research, Šalata 12, 10000, Zagreb, Croatia., Jurašin DD; Division of Physical Chemistry, Ruđer Bošković Institute, Bijenička cesta 54, 10 000, Zagreb, Croatia., Pavičić I; Institute for Medical Research and Occupational Health, Ksaverska cesta 2, 10000, Zagreb, Croatia., Marjanović Čermak AM; Institute for Medical Research and Occupational Health, Ksaverska cesta 2, 10000, Zagreb, Croatia., Milić M; Institute for Medical Research and Occupational Health, Ksaverska cesta 2, 10000, Zagreb, Croatia., Gajović S; University of Zagreb School of Medicine, Croatian Institute for Brain Research, Šalata 12, 10000, Zagreb, Croatia., Vinković Vrček I; Institute for Medical Research and Occupational Health, Ksaverska cesta 2, 10000, Zagreb, Croatia. Electronic address: ivinkovic@imi.hr.
Jazyk: angličtina
Zdroj: Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS) [J Trace Elem Med Biol] 2018 Dec; Vol. 50, pp. 684-692. Date of Electronic Publication: 2017 Dec 16.
DOI: 10.1016/j.jtemb.2017.12.003
Abstrakt: The rapid development and widespread applications of nanotechnology necessitates the design towards safe nanoparticles. Surface structure is among the most important physicochemical characteristics of metallic nanoparticles affecting their mode of action in certain biological or environmental compartments. This study aimed to investigate how different surface coatings affect the cytotoxicity and cellular uptake of silver nanoparticles (AgNPs) in murine neural stem cells (mNSCs). Different AgNPs were prepared by stabilisation with surface coatings encompassing sodium bis(2-ethylhexyl)-sulfosuccinate (AOT), cetyltrimethylammonium bromide (CTAB), poly(vinylpyrrolidone) (PVP), poly-l-lysine (PLL), and bovine serum albumin (BSA). The obtained results revealed that AgNPs stabilized with different surface coating caused different cytotoxicity effects and internalization pattern in mNSCs. Macropinocytosis was determined as the main uptake mechanism in mNSCs for all of the tested AgNP types. These findings contribute to the overall knowledge essential to the safety assessment of novel nanomaterials.
(Copyright © 2017 Elsevier GmbH. All rights reserved.)
Databáze: MEDLINE
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