Chronic Periodontitis and RANKL/OPG Ratio in Peri-Implant Mucosae Inflammation.

Autor: Costa LC; Postgraduate Program in Dentistry, UFF - Universidade Federal Fluminense, Niterói, RJ, Brazil., Fonseca MAD; Postgraduate Program in Dentistry, UFF - Universidade Federal Fluminense, Niterói, RJ, Brazil., Pinheiro ADR; Postgraduate Program in Oral Implantology, UFF - Universidade Federal Fluminense, Niterói, RJ, Brazil., Aguiar TRDS; Department of Periodontology, UFF - Universidade Federal Fluminense, Niterói, RJ, Brazil., Machado AN; Postgraduate Program in Oral Implantology, UFF - Universidade Federal Fluminense, Niterói, RJ, Brazil.; Department of Dental Clinical, UFF - Universidade Federal Fluminense, Niterói, RJ, Brazil., Quinelato V; Postgraduate Program in Dentistry, UFF - Universidade Federal Fluminense, Niterói, RJ, Brazil., Bonato LL; Postgraduate Program in Dentistry, UFF - Universidade Federal Fluminense, Niterói, RJ, Brazil., Aguiar DP; Orthopaedic and Traumatology National Institute, Rio de Janeiro, RJ, Brazil., Vieira T; Clinical Research Unit and Biology Institute, UFF - Universidade Federal Fluminense, Niterói, RJ, Brazil., Almeida FLD; Implantologist in Private Practice, Rio de Janeiro, RJ, Brazil., Lobo JC; Clinical Research Unit and Biology Institute, UFF - Universidade Federal Fluminense, Niterói, RJ, Brazil., Jordão M; Department of Oral Diagnosis, UFF - Universidade Federal Fluminense, Niterói, RJ, Brazil., Lomardo PG; Postgraduate Program in Dentistry, UFF - Universidade Federal Fluminense, Niterói, RJ, Brazil., Granjeiro JM; National Institute of Metrology, Quality and Technology, Rio de Janeiro, RJ, Brazil.; Department of Periodontology, UFF - Universidade Federal Flum inense, Niterói, RJ, Brazil., Casado PL; Postgraduate Program in Dentistry, UFF - Universidade Federal Fluminense, Niterói, RJ, Brazil.; Cell Therapy Center, Clinical Research Unit and Biology Institute, UFF - Universidade Federal Fluminense, Niterói, RJ, Brazil.
Jazyk: angličtina
Zdroj: Brazilian dental journal [Braz Dent J] 2018 Jan-Feb; Vol. 29 (1), pp. 14-22.
DOI: 10.1590/0103-6440201801241
Abstrakt: tHistory of chronic periodontitis (CP) is a risk factor for oseointegration failure. The osteoclastogenesis system (RANK, RANKL and OPG) is critical for bone homeostatic control. We investigated the levels of OPG and RANKL in peri-implant tissues from volunteers with and without a history of CP and their association with mucosae inflammation. This is a single-blind case-contro study. Diagnosis of a history of CP and peri-implant examination was performed on 46 volunteers, divided into control (without history of CP, n=26) and CP group (with history of CP, n=20). Gingival biopsies were harvested during implant exposure. Quantitative PCR evaluated OPG/RANKL mRNA expressions. OPG and RANKL proteins were analyzed by western blot and immunohistochemistry assay. The chi-square test analyzed the significance of nominal variables between groups while continuous variables were analyzed by T-test or Mann-Whitney test, after Shapiro-Wilk test evaluation. The 2-ΔΔCT Livak method calculation evaluated the gene expression. Values of p<0.05 were considered statistically significant. Volunteers with CP history had 23 times higher chance of developing mucosae inflammation. High mucosae levels of RANKL (p=0.04) and RANKL/OPG (p=0.001) mRNA expressions were observed in CP group. CP volunteers showed increased RANKL protein levels in opposition to decreased OPG expression. Even without active periodontitis, volunteers with a history of CP had elevated gingival levels of RANKL/OPG and higher correlation with peri-implant mucosae inflammation and implant loss.
Databáze: MEDLINE
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