| Autor: |
Burmenskaya OV; a Federal State Budget Institution 'Research Center for Obstetrics, Gynecology and Perinatology' Ministry of Healthcare of the Russian Federation , Moscow , Russia., Bozhenko VK; a Federal State Budget Institution 'Research Center for Obstetrics, Gynecology and Perinatology' Ministry of Healthcare of the Russian Federation , Moscow , Russia., Smolnikova VY; a Federal State Budget Institution 'Research Center for Obstetrics, Gynecology and Perinatology' Ministry of Healthcare of the Russian Federation , Moscow , Russia., Kalinina EA; a Federal State Budget Institution 'Research Center for Obstetrics, Gynecology and Perinatology' Ministry of Healthcare of the Russian Federation , Moscow , Russia., Korneeva IE; a Federal State Budget Institution 'Research Center for Obstetrics, Gynecology and Perinatology' Ministry of Healthcare of the Russian Federation , Moscow , Russia., Donnikov AE; a Federal State Budget Institution 'Research Center for Obstetrics, Gynecology and Perinatology' Ministry of Healthcare of the Russian Federation , Moscow , Russia., Beyk ЕP; a Federal State Budget Institution 'Research Center for Obstetrics, Gynecology and Perinatology' Ministry of Healthcare of the Russian Federation , Moscow , Russia., Naumov VA; a Federal State Budget Institution 'Research Center for Obstetrics, Gynecology and Perinatology' Ministry of Healthcare of the Russian Federation , Moscow , Russia., Aleksandrova NV; a Federal State Budget Institution 'Research Center for Obstetrics, Gynecology and Perinatology' Ministry of Healthcare of the Russian Federation , Moscow , Russia., Borovikov PI; a Federal State Budget Institution 'Research Center for Obstetrics, Gynecology and Perinatology' Ministry of Healthcare of the Russian Federation , Moscow , Russia., Trofimov DY; a Federal State Budget Institution 'Research Center for Obstetrics, Gynecology and Perinatology' Ministry of Healthcare of the Russian Federation , Moscow , Russia. |
| Abstrakt: |
To determine the most informative markers for assessing the functional state of endometrium during the 'window of implantation' and creating a model for assessment of the readiness of endometrium for embryo implantation. Forty-seven women with tubal infertility and a successful IVF pregnancy participated in the study. Pipelle endometrial sample was performed during the supposed 'window of implantation' in natural cycle with subsequent histological study, and transcriptional profile of genes GPX3, PAEP, DPP4, TAGLN, HABP2, IMPA2, AQP3, HLA-DOB, MSX1, POSTN determined by real-time quantitative polymerase chain reaction (qRT-PCR). Differences in the level of mRNA expression of all the studied genes in the receptive endometrium were found in comparison to the prereceptive one, which allowed us to classify two functional states of the endometrium. The results of histological examination responded to the stage of maturation of the endometrium in 78.7% of cases. Receptive endometrial status can be determined based on the integral evaluation of mRNA expression level of 4 PAEP, DPP4, MSX1, and HLA-DOB genes. The model for determining a personalized `window implantation' is offered for practical application in ART. |
