PEGylation of zinc nanoparticles amplifies their ability to enhance olfactory responses to odorant.

Autor: Singletary M; Department of Anatomy, Physiology and Pharmacology, Auburn University College of Veterinary Medicine, Auburn, Alabama, United States of America., Hagerty S; Department of Anatomy, Physiology and Pharmacology, Auburn University College of Veterinary Medicine, Auburn, Alabama, United States of America., Muramoto S; Material Measurement Laboratory, National Institute of Standards and Technology, Gaithersburg, Maryland, United States of America., Daniels Y; Material Measurement Laboratory, National Institute of Standards and Technology, Gaithersburg, Maryland, United States of America., MacCrehan WA; Material Measurement Laboratory, National Institute of Standards and Technology, Gaithersburg, Maryland, United States of America., Stan G; Material Measurement Laboratory, National Institute of Standards and Technology, Gaithersburg, Maryland, United States of America., Lau JW; Material Measurement Laboratory, National Institute of Standards and Technology, Gaithersburg, Maryland, United States of America., Pustovyy O; Department of Anatomy, Physiology and Pharmacology, Auburn University College of Veterinary Medicine, Auburn, Alabama, United States of America., Globa L; Department of Anatomy, Physiology and Pharmacology, Auburn University College of Veterinary Medicine, Auburn, Alabama, United States of America., Morrison EE; Department of Anatomy, Physiology and Pharmacology, Auburn University College of Veterinary Medicine, Auburn, Alabama, United States of America., Sorokulova I; Department of Anatomy, Physiology and Pharmacology, Auburn University College of Veterinary Medicine, Auburn, Alabama, United States of America., Vodyanoy V; Department of Anatomy, Physiology and Pharmacology, Auburn University College of Veterinary Medicine, Auburn, Alabama, United States of America.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2017 Dec 20; Vol. 12 (12), pp. e0189273. Date of Electronic Publication: 2017 Dec 20 (Print Publication: 2017).
DOI: 10.1371/journal.pone.0189273
Abstrakt: Olfactory responses are intensely enhanced with the addition of endogenous and engineered primarily-elemental small zinc nanoparticles (NPs). With aging, oxidation of these Zn nanoparticles eliminated the observed enhancement. The design of a polyethylene glycol coating to meet storage requirements of engineered zinc nanoparticles is evaluated to achieve maximal olfactory benefit. The zinc nanoparticles were covered with 1000 g/mol or 400 g/mol molecular weight polyethylene glycol (PEG). Non-PEGylated and PEGylated zinc nanoparticles were tested by electroolfactogram with isolated rat olfactory epithelium and odorant responses evoked by the mixture of eugenol, ethyl butyrate and (±) carvone after storage at 278 K (5 oC), 303 K (30 oC) and 323 K (50 oC). The particles were analyzed by atomic force microscopy, transmission electron microscopy, X-ray photoelectron spectroscopy, and laser Doppler velocimetry. Our data indicate that stored ZnPEG400 nanoparticles maintain physiologically-consistent olfactory enhancement for over 300 days. These engineered Nanoparticles support future applications in olfactory research, sensitive detection, and medicine.
Databáze: MEDLINE