The effects of platelet lysate on maturation, fertilization and embryo development of NMRI mouse oocytes at germinal vesicle stage.

Autor: Pazoki H; Department of Embryology at Reproductive Biomedicine Research Center Royan Institute for Reproductive Biomedicine, ACECR Tehran Iran.; Department of Biology, Faculty of Science Urmia University Urmia Iran., Eimani H; Department of Embryology at Reproductive Biomedicine Research Center Royan Institute for Reproductive Biomedicine, ACECR Tehran Iran.; Department of Anatomy, Faculty of Medicine Baqyiatallah (a.s.) University of Medical Sciences Tehran Iran., Farokhi F; Department of Biology, Faculty of Science Urmia University Urmia Iran., Shahverdi AH; Department of Embryology at Reproductive Biomedicine Research Center Royan Institute for Reproductive Biomedicine, ACECR Tehran Iran., Tahaei LS; Department of Embryology at Reproductive Biomedicine Research Center Royan Institute for Reproductive Biomedicine, ACECR Tehran Iran.
Jazyk: angličtina
Zdroj: Reproductive medicine and biology [Reprod Med Biol] 2015 Aug 08; Vol. 15 (2), pp. 115-120. Date of Electronic Publication: 2015 Aug 08 (Print Publication: 2016).
DOI: 10.1007/s12522-015-0220-x
Abstrakt: Purpose: Improving in vitro maturation could increase the rate of pregnancy from oocytes matured in vitro. Consequently, patients will be prevented from using gonadotropin with its related side effects. In this study, the maturation medium was enriched by platelet lysate (PL), then maturation and subsequent developments were monitored.
Methods: Oocytes at germinal vesicle stage with cumulus cells (cumulus-oocyte complex) and without cumulus cells (denuded oocytes) were obtained from mature female mice. The maturation medium was enriched by 5 and 10 % PL and 5 % PL + 5 % fetal bovine serum (FBS) as experimental groups; the control groups' media consisted of 5 and 10 % FBS. After 18 h, the matured oocytes were collected and, after fertilization, subsequent development was monitored.
Results: The rates of maturation, fertilization and 2-cell embryo development for the denuded oocyte groups in experimental media 5 % PL and 5 % PL + 5 % FBS were significantly higher than those of the control groups ( P < 0.05), while the results for the cumulus-oocyte complex groups were similar between the experimental groups and control groups.
Conclusions: The results of this study indicated that platelet lysate could improve the maturation rate in the absence of granulosa cells compared to media with FBS. This extract also had positive effects on fertilization and embryo development.
Competing Interests: Hassan Pazoki, Dr Hussein Eimani, Dr Farah Farokhi, Dr Abdol Hossein Shahverdi and Leila Sadat Tahaei declare that they have no conflict of interest.
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje