[Effects of lncRNA RP11-770J1.3 and TMEM25 expression on paclitaxel resistance in human breast cancer cells].

Autor:	Li Y; Clinical Testing and Diagnose Experimental Center, Bengbu Medical College, Bengbu 233000, China., Wang Y; Clinical Testing and Diagnose Experimental Center, Bengbu Medical College, Bengbu 233000, China., Wang H; Clinical Testing and Diagnose Experimental Center, Bengbu Medical College, Bengbu 233000, China., Zhang L; Clinical Testing and Diagnose Experimental Center, Bengbu Medical College, Bengbu 233000, China., Ding Y; Department of Oncology, the Third People's Hospital of Bengbu, Bengbu 233000, China., Chen S; Department of Biochemistry & Molecular Biology, Bengbu Medical College, Bengbu 233000, China., Yang Q; Department of Biochemistry & Molecular Biology, Bengbu Medical College, Bengbu 233000, China., Chen C; Department of Biochemistry & Molecular Biology, Bengbu Medical College, Bengbu 233000, China. tochenchangjie@163.com.
Jazyk:	čínština
Zdroj:	Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences [Zhejiang Da Xue Xue Bao Yi Xue Ban] 2017 Jul 25; Vol. 46 (4), pp. 364-370.
DOI:	10.3785/j.issn.1008-9292.2017.08.04
Abstrakt:	Objective: To investigate the effects of long non-coding RNA(lncRNA) RP11-770J1.3 and transmembrane protein 25 (TMEM25) on paclitaxel resistance in human breast cancer MCF-7/PR cell line. Methods: The expression of lncRNA RP11-770J1.3 and TMEM25 in human breast cancer MCF-7(paclitaxel sensitive) and MCF-7/PR(paclitaxel resistant) cells were detected by quantitative RT-PCR. The synthetic interfering fragments of lncRNA RP11-770J1.3 and TMEM25 were transfected into MCF-7/PR cells. Sulforhodamine B assay was used to detect the sensitivity of MCF-7/PR cells to paclitaxel after interference of lncRNA RP11-770J1.3 and TMEM25. The expression of multidrug-resistance genes and proteins were detected by qRT-PCR and Western blot, respectively. Results: lncRNA RP11-770J1.3 and TMEM25 were highly expressed in MCF-7/PR cells, and were significantly down-regulated after transfection of synthetic interfering fragments. Down-regulation of lncRNA RP11-770J1.3 and TMEM25 enhanced the sensitivity of MCF-7/PR cells to paclitaxel, and inhibited the expression of MRP, BCRP and MDR1/P-gp (all P <0.05). Such effects were more significant when lncRNA RP11-770J1.3 and TMEM25 were both down-regulated (all P <0.05). Conclusions: lncRNA RP11-770J1.3 and TMEM25 are highly expressed in MCF-7/PR cells, and the down-regulation of lncRNA RP11-770J1.3 and TMEM25 can enhance paclitaxel sensitivity in MCF-7/PR cells.
Databáze:	MEDLINE
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