| Autor: |
Premarathne JMKJK; Faculty of Food Science and Technology, Food Safety Research Center, Universiti Putra Malaysia, Seri Kembangan, Malaysia.; Department of Livestock and Avian Science, Faculty of Livestock, Fisheries and Nutrition, Wayamba University of Sri Lanka, Kuliyapitiya, Sri Lanka., Anuar AS; Faculty of Food Science and Technology, Food Safety Research Center, Universiti Putra Malaysia, Seri Kembangan, Malaysia., Thung TY; Faculty of Food Science and Technology, Food Safety Research Center, Universiti Putra Malaysia, Seri Kembangan, Malaysia., Satharasinghe DA; Institute of Bio Science, Universiti Putra Malaysia, Seri Kembangan, Malaysia.; Department of Basic Veterinary Science, Faculty of Veterinary Medicine and Animal Science, University of Peradeniya, Peradeniya, Sri Lanka., Jambari NN; Food Safety Research Center (FOSREC), Faculty of Food Science and Technology, Universiti Putra Malaysia, Seri Kembangan, Malaysia., Abdul-Mutalib NA; Food Safety Research Center (FOSREC), Faculty of Food Science and Technology, Universiti Putra Malaysia, Seri Kembangan, Malaysia., Huat JTY; Faculty of Food Technology, Universiti Sultan Zainal Abidin, Kuala Terengganu, Malaysia., Basri DF; Faculty of Health Sciences, School of Diagnostic and Applied Health Sciences, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia., Rukayadi Y; Faculty of Food Science and Technology, Food Safety Research Center, Universiti Putra Malaysia, Seri Kembangan, Malaysia., Nakaguchi Y; Center for Southeast Asian Studies, Kyoto University, Kyoto, Japan., Nishibuchi M; Center for Southeast Asian Studies, Kyoto University, Kyoto, Japan., Radu S; Faculty of Food Science and Technology, Food Safety Research Center, Universiti Putra Malaysia, Seri Kembangan, Malaysia.; Laboratory of Food Safety and Food Integrity, Institute of Tropical Agriculture and Food Security, Universiti Putra Malaysia, Seri Kembangan, Malaysia. |
| Abstrakt: |
Campylobacter is a major foodborne pathogen frequently associated with human bacterial gastroenteritis in the world. This study was conducted to determine the prevalence and antibiotic resistance of Campylobacter spp. in the beef food system in Malaysia. A total of 340 samples consisting of cattle feces ( n = 100), beef ( n = 120) from wet markets and beef ( n = 120) from hypermarkets were analyzed for Campylobacter spp. The overall prevalence of Campylobacter was 17.4%, consisting of 33% in cattle fecal samples, 14.2% in raw beef from wet market and 7.5% in raw beef from the hypermarket. The multiplex-polymerase chain reaction (PCR) identified 55% of the strains as C. jejuni , 26% as C. coli , and 19% as other Campylobacter spp. A high percentage of Campylobacter spp. were resistant to tetracycline (76.9%) and ampicillin (69.2%), whilst low resistance was exhibited to chloramphenicol (7.6%). The MAR Index of Campylobacter isolates from this study ranged from 0.09 to 0.73. The present study indicates the potential public health risk associated with the beef food system, hence stringent surveillance, regulatory measures, and appropriate interventions are required to minimize Campylobacter contamination and prudent antibiotic usage that can ensure consumer safety. |
