Organic matter processing by microbial communities throughout the Atlantic water column as revealed by metaproteomics.

Autor:	Bergauer K; Department of Limnology and Bio-Oceanography, University of Vienna, A-1090 Vienna, Austria; kristin.bergauer@univie.ac.at., Fernandez-Guerra A; Microbial Genomics and Bioinformatics Research Group, Max Planck Institute for Marine Microbiology, D-28359 Bremen, Germany.; Oxford e-Research Centre, University of Oxford, Oxford OX1 3QG, United Kingdom., Garcia JAL; Department of Limnology and Bio-Oceanography, University of Vienna, A-1090 Vienna, Austria., Sprenger RR; Department of Biochemistry and Molecular Biology, VILLUM Center for Bioanalytical Sciences, University of Southern Denmark, DK-5230 Odense M, Denmark., Stepanauskas R; Bigelow Laboratory for Ocean Sciences, East Boothbay, ME 04544., Pachiadaki MG; Bigelow Laboratory for Ocean Sciences, East Boothbay, ME 04544., Jensen ON; Department of Biochemistry and Molecular Biology, VILLUM Center for Bioanalytical Sciences, University of Southern Denmark, DK-5230 Odense M, Denmark., Herndl GJ; Department of Limnology and Bio-Oceanography, University of Vienna, A-1090 Vienna, Austria.; Department of Marine Microbiology and Biogeochemistry, Royal Netherlands Institute for Sea Research, Utrecht University, 1790 AB Den Burg, The Netherlands.; Vienna Metabolomics Center, University of Vienna, A-1090 Vienna, Austria.
Jazyk:	angličtina
Zdroj:	Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2018 Jan 16; Vol. 115 (3), pp. E400-E408. Date of Electronic Publication: 2017 Dec 18.
DOI:	10.1073/pnas.1708779115
Abstrakt:	The phylogenetic composition of the heterotrophic microbial community is depth stratified in the oceanic water column down to abyssopelagic layers. In the layers below the euphotic zone, it has been suggested that heterotrophic microbes rely largely on solubilized particulate organic matter as a carbon and energy source rather than on dissolved organic matter. To decipher whether changes in the phylogenetic composition with depth are reflected in changes in the bacterial and archaeal transporter proteins, we generated an extensive metaproteomic and metagenomic dataset of microbial communities collected from 100- to 5,000-m depth in the Atlantic Ocean. By identifying which compounds of the organic matter pool are absorbed, transported, and incorporated into microbial cells, intriguing insights into organic matter transformation in the deep ocean emerged. On average, solute transporters accounted for 23% of identified protein sequences in the lower euphotic and ∼39% in the bathypelagic layer, indicating the central role of heterotrophy in the dark ocean. In the bathypelagic layer, substrate affinities of expressed transporters suggest that, in addition to amino acids, peptides and carbohydrates, carboxylic acids and compatible solutes may be essential substrates for the microbial community. Key players with highest expression of solute transporters were Alphaproteobacteria, Gammaproteobacteria, and Deltaproteobacteria, accounting for 40%, 11%, and 10%, respectively, of relative protein abundances. The in situ expression of solute transporters indicates that the heterotrophic prokaryotic community is geared toward the utilization of similar organic compounds throughout the water column, with yet higher abundances of transporters targeting aromatic compounds in the bathypelagic realm. Competing Interests: The authors declare no conflict of interest. (Copyright © 2018 the Author(s). Published by PNAS.)
Databáze:	MEDLINE
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