Two types of lysozymes from the whitefly Bemisia tabaci: Molecular characterization and functional diversification.

Autor: Wang ZZ; State Key Lab of Rice Biology and Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insect Pests, Institute of Insect Sciences, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, China., Zhan LQ; State Key Lab of Rice Biology and Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insect Pests, Institute of Insect Sciences, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, China., Chen XX; State Key Lab of Rice Biology and Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insect Pests, Institute of Insect Sciences, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, China. Electronic address: xxchen@zju.edu.cn.
Jazyk: angličtina
Zdroj: Developmental and comparative immunology [Dev Comp Immunol] 2018 Apr; Vol. 81, pp. 252-261. Date of Electronic Publication: 2017 Dec 13.
DOI: 10.1016/j.dci.2017.12.012
Abstrakt: Lysozyme is well-known as an immune effector in the immune system. Here we identified three genes including one c-type lysozyme, Btlysc, and two i-type lysozymes, Btlysi1 and Btlysi2, from the whitefly Bemisia tabaci. All three lysozymes were constitutively expressed in different tissues and developmental stages, but the two types of lysozymes showed different expression patterns. The expression levels of Btlysi1 and Btlysi2 were dramatically induced after the whitefly fed with different host plants while the expression level of Btlysc kept unchanged. After fungal infection and begomovirus acquisition, Btlysc expression was significantly upregulated while Btlysi1 and Btlysi2 expression were basically not induced. Furthermore, we found that Btlysc showed muramidase and antibacterial activities. Altogether, our results suggest that the two types of lysozymes act in two different ways in B. tabaci, that is, Btlysc is involved in the whitefly immune system while Btlysi1 and Btlysi2 may play a role in digestion or nutrition absorption.
(Copyright © 2017 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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