The second molecular epidemiological study of HIV infection in Mongolia between 2010 and 2016.

Autor: Jagdagsuren D; AIDS/STI Surveillance and Research Department, National Center for Communicable Diseases, Ulaanbaatar, Mongolia., Hayashida T; AIDS Clinical Center, National Center for Global Health and Medicine, Tokyo, Japan., Takano M; AIDS Clinical Center, National Center for Global Health and Medicine, Tokyo, Japan., Gombo E; AIDS/STI Surveillance and Research Department, National Center for Communicable Diseases, Ulaanbaatar, Mongolia., Zayasaikhan S; AIDS/STI Surveillance and Research Department, National Center for Communicable Diseases, Ulaanbaatar, Mongolia., Kanayama N; AIDS Clinical Center, National Center for Global Health and Medicine, Tokyo, Japan., Tsuchiya K; AIDS Clinical Center, National Center for Global Health and Medicine, Tokyo, Japan., Oka S; AIDS Clinical Center, National Center for Global Health and Medicine, Tokyo, Japan.; Center for AIDS Research, Kumamoto University, Kumamoto, Japan.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2017 Dec 15; Vol. 12 (12), pp. e0189605. Date of Electronic Publication: 2017 Dec 15 (Print Publication: 2017).
DOI: 10.1371/journal.pone.0189605
Abstrakt: Objective: Our previous 2005-2009 molecular epidemiological study in Mongolia identified a hot spot of HIV-1 transmission in men who have sex with men (MSM). To control the infection, we collaborated with NGOs to promote safer sex and HIV testing since mid-2010. In this study, we carried out the second molecular epidemiological survey between 2010 and 2016 to determine the status of HIV-1 infection in Mongolia.
Methods: The study included 143 new cases of HIV-1 infection. Viral RNA was extracted from stocked plasma samples and sequenced for the pol and the env regions using the Sanger method. Near-full length sequencing using MiSeq was performed in 3 patients who were suspected to be infected with recombinant HIV-1. Phylogenetic analysis was performed using the neighbor-joining method and Bayesian Markov chain Monte Carlo method.
Results: MSM was the main transmission route in the previous and current studies. However, heterosexual route showed a significant increase in recent years. Phylogenetic analysis documented three taxa; Mongolian B, Korean B, and CRF51_01B, though the former two were also observed in the previous study. CRF51_01B, which originated from Singapore and Malaysia, was confirmed by near-full length sequencing. Although these strains were mainly detected in MSM, they were also found in increasing numbers of heterosexual males and females. Bayesian phylogenetic analysis estimated transmission of CRF51_01B into Mongolia around early 2000s. An extended Bayesian skyline plot showed a rapid increase in the effective population size of Mongolian B cluster around 2004 and that of CRF51_01B cluster around 2011.
Conclusions: HIV-1 infection might expand to the general population in Mongolia. Our study documented a new cluster of HIV-1 transmission, enhancing our understanding of the epidemiological status of HIV-1 in Mongolia.
Databáze: MEDLINE
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