| Autor: |
Ten Brinke MM; Department of Neuroscience, Erasmus Medical Center, Rotterdam, Netherlands., Heiney SA; Department of Neuroscience, Baylor College of Medicine, Houston, United States., Wang X; Department of Neuroscience, Erasmus Medical Center, Rotterdam, Netherlands., Proietti-Onori M; Department of Neuroscience, Erasmus Medical Center, Rotterdam, Netherlands., Boele HJ; Department of Neuroscience, Erasmus Medical Center, Rotterdam, Netherlands., Bakermans J; Department of Neuroscience, Erasmus Medical Center, Rotterdam, Netherlands., Medina JF; Department of Neuroscience, Baylor College of Medicine, Houston, United States., Gao Z; Department of Neuroscience, Erasmus Medical Center, Rotterdam, Netherlands., De Zeeuw CI; Department of Neuroscience, Erasmus Medical Center, Rotterdam, Netherlands.; Netherlands Institute for Neuroscience, Royal Academy of Arts and Sciences (KNAW), Amsterdam, Netherlands. |
| Abstrakt: |
While research on the cerebellar cortex is crystallizing our understanding of its function in learning behavior, many questions surrounding its downstream targets remain. Here, we evaluate the dynamics of cerebellar interpositus nucleus (IpN) neurons over the course of Pavlovian eyeblink conditioning. A diverse range of learning-induced neuronal responses was observed, including increases and decreases in activity during the generation of conditioned blinks. Trial-by-trial correlational analysis and optogenetic manipulation demonstrate that facilitation in the IpN drives the eyelid movements. Adaptive facilitatory responses are often preceded by acquired transient inhibition of IpN activity that, based on latency and effect, appear to be driven by complex spikes in cerebellar cortical Purkinje cells. Likewise, during reflexive blinks to periocular stimulation, IpN cells show excitation-suppression patterns that suggest a contribution of climbing fibers and their collaterals. These findings highlight the integrative properties of subcortical neurons at the cerebellar output stage mediating conditioned behavior. |
