Antagonists of Wnt/β-catenin signalling in the periodontitis associated with type 2 diabetes and smoking.

Autor: Miranda TS; Dental Research Division, Department of Periodontology, Guarulhos University, São Paulo, Brazil., Napimoga MH; Laboratory of Immunology and Molecular Biology, São Leopoldo Mandic Institute and Research Center, Campinas, Brazil., Feres M; Dental Research Division, Department of Periodontology, Guarulhos University, São Paulo, Brazil., Marins LM; Dental Research Division, Department of Periodontology, Guarulhos University, São Paulo, Brazil., da Cruz DF; Dental Research Division, Department of Periodontology, Guarulhos University, São Paulo, Brazil., da Silva HDP; Dental Research Division, Department of Periodontology, Guarulhos University, São Paulo, Brazil., Duarte PM; Dental Research Division, Department of Periodontology, Guarulhos University, São Paulo, Brazil.
Jazyk: angličtina
Zdroj: Journal of clinical periodontology [J Clin Periodontol] 2018 Mar; Vol. 45 (3), pp. 293-302. Date of Electronic Publication: 2018 Jan 12.
DOI: 10.1111/jcpe.12854
Abstrakt: Aim: This study evaluated the levels of sclerostin (SOST) and Dickkopf (DKK)-1 in the chronic periodontitis (CP) associated with type 2 diabetes (DM) and/or smoking. Relationships between SOST, DDK1, RANKL, OPG, IL-1ß, IL-6 and TNF-α, and pathogens were assessed.
Material and Methods: The study population included non-diabetic non-smokers (control), non-smokers with DM (DM group), non-diabetic smokers (S group) and smokers with DM (SDM group), all with CP. Serum and gingival levels of SOST, DKK1, RANKL, OPG, IL-1ß, IL-6 and TNF-α were evaluated by multiplex immunoassay. Gene expressions of these biomarkers and subgingival levels of pathogens were assessed by qPCR.
Results: Gingival protein and/or mRNA levels of DKK1 and SOST were higher in subjects with DM and/or smoking than in controls (p < .05). Serum levels of SOST were higher in the DM group than in controls (p < .05). DKK1 positively correlated with SOST in the DM, SDM and control groups (p < .05) at mRNA levels. DKK-1 and SOST correlated with pathogens, especially in both groups with DM.
Conclusions: SOST and DKK1 were upregulated in patients with CP presenting DM and/or smoking. DM, alone or with smoking, particularly influenced the correlations of SOST and DKK1 with each other and with the other biomarkers mostly at mRNA levels, as well as with periodontal pathogens.
(© 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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