A Coxiella mutualist symbiont is essential to the development of Rhipicephalus microplus.

Autor: Guizzo MG; Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil., Parizi LF; Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil., Nunes RD; Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil., Schama R; Laboratório de Biologia Computacional e Sistemas, IOC - Fiocruz, Rio de Janeiro, RJ, Brazil., Albano RM; Departamento de Bioquímica, IBRAG, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, RJ, Brazil., Tirloni L; Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil., Oldiges DP; Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil., Vieira RP; Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil., Oliveira WHC; Departamento de Química, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ, Brazil., Leite MS; Departamento de Química, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ, Brazil., Gonzales SA; Instituto de Biotecnologia, Centro de Investigaciones en Ciencias Veterinarias y Agronómicas, INTA, Buenos Aires, Argentina., Farber M; Instituto de Biotecnologia, Centro de Investigaciones en Ciencias Veterinarias y Agronómicas, INTA, Buenos Aires, Argentina., Martins O; Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil., Vaz IDS Jr; Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.; Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.; Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular (INCT-EM), Rio de Janeiro, RJ, Brazil., Oliveira PL; Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil. pedro@bioqmed.ufrj.br.; Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular (INCT-EM), Rio de Janeiro, RJ, Brazil. pedro@bioqmed.ufrj.br.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2017 Dec 14; Vol. 7 (1), pp. 17554. Date of Electronic Publication: 2017 Dec 14.
DOI: 10.1038/s41598-017-17309-x
Abstrakt: The cattle tick Rhipicephalus microplus is a hematophagous ectoparasite that causes important economic losses in livestock. Different species of ticks harbor a symbiont bacterium of the genus Coxiella. It was showed that a Coxiella endosymbiont from R. microplus (CERM) is a vertically transmitted mutualist symbiont, comprising 98% of the 16S rRNA sequences in both eggs and larvae. Sequencing of the bacterial genome revealed genes for biosynthetic pathways for several vitamins and key metabolic cofactors that may provide a nutritional complement to the tick host. The CERM was abundant in ovary and Malpighian tubule of fully engorged female. Tetracycline treatment of either the tick or the vertebrate host reduced levels of bacteria in progeny in 74% for eggs and 90% for larvae without major impact neither on the reproductive fitness of the adult female or on embryo development. However, CERM proved to be essential for the tick to reach the adult life stage, as under antibiotic treatment no tick was able to progress beyond the metanymph stage. Data presented here suggest that interference in the symbiotic CERM-R. microplus relationship may be useful to the development of alternative control methods, highlighting the interdependence between ticks and their endosymbionts.
Databáze: MEDLINE
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