Dechorionation and Permeabilization of Podisus nigrispinus (Heteroptera: Pentatomidae) Eggs: Limiting Factors for Cryopreservation.

Autor:	Dos Santos ÍTBF; Universidade Federal de Sergipe, Laboratório de Entomologia Florestal, Departamento de Ciências Florestais, Jardim Rosa Elze, São Cristóvão-SE., Poderoso JCM; Universidade Federal de Sergipe, Laboratório de Entomologia Florestal, Departamento de Ciências Florestais, Jardim Rosa Elze, São Cristóvão-SE., Dos Santos EJS; Universidade Federal de Sergipe, Laboratório de Entomologia Florestal, Departamento de Ciências Florestais, Jardim Rosa Elze, São Cristóvão-SE., Ribeiro GT; Universidade Federal de Sergipe, Laboratório de Entomologia Florestal, Departamento de Ciências Florestais, Jardim Rosa Elze, São Cristóvão-SE., Cossolin JFS; Universidade Federal de Viçosa, Laboratório de Ultra Estrutura Celular, Departamento de Biologia Geral, Viçosa-MG., Serrão JE; Universidade Federal de Viçosa, Laboratório de Ultra Estrutura Celular, Departamento de Biologia Geral, Viçosa-MG.
Jazyk:	angličtina
Zdroj:	Journal of economic entomology [J Econ Entomol] 2018 Feb 09; Vol. 111 (1), pp. 96-100.
DOI:	10.1093/jee/tox313
Abstrakt:	Cryopreservation protocols have been developed for eggs of Diptera, Lepidoptera, and Hymenoptera; however, for Heteroptera, such as Podisus nigrispinus Dallas, 1851 (Pentatomidae), no procedures have been described yet. The objective of this study was to evaluate the processes of dechorionation and permeabilization on the viability of eggs of P. nigrispinus with different embryonic ages. In the laboratory, embryos of 24, 48, 72, and 96 h of age were submitted to sodium hydroxide, sodium hypochlorite, isopropyl, and hexane solutions for dechorionation and permeabilization. The experiment was carried out in a 4 × 8 factorial scheme. Sodium hydroxide affects embryo viability; however, 96-h-old embryos showed higher viability when compared with 24, 48, and 72-h-old embryos. Microscope observations showed that, after the treatments, the chorion of P. nigrispinus eggs was reduced to 5.11 ± 0.30 µm. These solutions for dechorionation and permeabilization of P. nigrispinus eggs together with embryonic age affect the viability of embryos to be cryopreserved. (© The Author(s) 2017. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
Databáze:	MEDLINE
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