Effect of 670 nm laser photobiomodulation on vascular density and fibroplasia in late stages of tissue repair.

Autor: Fortuna T; Basic Science Department, Bahiana School of Medicine and Public Health, Salvador, Bahia, Brazil., Gonzalez AC; Experimental Pathology Laboratory, Gonçalo Moniz Research Center, FIOCRUZ, Salvador, Bahia, Brazil., Sá MF; Basic Science Department, Bahiana School of Medicine and Public Health, Salvador, Bahia, Brazil., Andrade ZA; Experimental Pathology Laboratory, Gonçalo Moniz Research Center, FIOCRUZ, Salvador, Bahia, Brazil., Reis SRA; Bahiana School of Medicine and Public Health, Salvador, Bahia, Brazil., Medrado ARAP; Bahiana School of Medicine and Public Health, Salvador, Bahia, Brazil.
Jazyk: angličtina
Zdroj: International wound journal [Int Wound J] 2018 Apr; Vol. 15 (2), pp. 274-282. Date of Electronic Publication: 2017 Dec 13.
DOI: 10.1111/iwj.12861
Abstrakt: This study aimed to investigate the effects of gallium-aluminum-arsenium (GaAlAs) (670 nm) laser therapy on neoangiogenesis and fibroplasia during tissue remodelling. Forty male Wistar rats underwent cutaneous surgery and were divided into 2 experimental groups: the Control and Laser group (9 mW, 670 nm, 0.031 W/cm 2 , 4 J/cm 2 ). After 14, 21, 28, and 35 days, the animals were euthanised. Descriptive and quantitative analyses were performed in sections stained with haematoxylin-eosin and Sirius Red, respectively. The amounts of VEGF+ and CD31+ cells were evaluated by immunohistochemistry and histomorphometric analysis, respectively. Statistical analysis was performed using the Mann-Whitney, Friedman, and Spearman correlation test, P < 0.05. The collagen expression was significantly higher in the laser group compared with the control group on days 14 and 21 after the creation of the skin wound (P = 0.008; P = 0.016) and in the control group between 14 and 28 and 14 and 35 days (P = 0.001; P = 0.007). There were more blood vessels in three periods of the study only in the (Laser) treated group, with statistical significance at day 14 (P = 0.016). There was no statistically significant difference in VEGF+ cell count in the different experimental groups throughout the study, although a positive correlation was shown with the area of collagen on days 14 and 28 (P = 0.037). Laser treatment had a positive effect in the late course of healing, particularly with regards to collagen expression and the number of newly formed vessels. VEGF+ cells were present in both experimental groups, and VEGF appeared to influence fibroplasia in the treated group.
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Databáze: MEDLINE
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