Induction of apoptosis in human glioma cell lines of various grades through the ROS-mediated mitochondrial pathway and caspase activation by Rhaponticum carthamoides transformed root extract.

Autor: Skała E; Department of Biology and Pharmaceutical Botany, Medical University of Łódź, Muszyńskiego 1, 90-151, Łódź, Poland. ewa.skala@umed.lodz.pl., Kowalczyk T; Department of Genetics and Plant Molecular Biology and Biotechnology, The University of Łódź, Banacha 12/13, 90-237, Łódź, Poland., Toma M; Laboratory of Medical Genetics, University of Łódź, Pomorska 141/143, 90-236, Łódź, Poland., Szemraj J; Department of Medical Biochemistry, Medical University of Łódź, Mazowiecka 6/8, 92-215, Łódź, Poland., Radek M; Department of Neurosurgery, Surgery of Spine and Peripheral Nerves, Medical University of Łódź, University Hospital WAM-CSW, Łódź, Poland., Pytel D; Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Hollings Cancer Center, HCC-709, 86 Jonathan Lucas Street, Charleston, SC, 29425, USA., Wieczfinska J; Department of Immunopathology, Chair of Allergology, Immunology and Dermatology, Faculty of Biomedical Sciences and Postgraduate Training, Medical University of Łódź, Żeligowskiego 7/9, 90-752, Łódź, Poland., Wysokińska H; Department of Biology and Pharmaceutical Botany, Medical University of Łódź, Muszyńskiego 1, 90-151, Łódź, Poland., Śliwiński T; Laboratory of Medical Genetics, University of Łódź, Pomorska 141/143, 90-236, Łódź, Poland., Sitarek P; Department of Biology and Pharmaceutical Botany, Medical University of Łódź, Muszyńskiego 1, 90-151, Łódź, Poland.
Jazyk: angličtina
Zdroj: Molecular and cellular biochemistry [Mol Cell Biochem] 2018 Aug; Vol. 445 (1-2), pp. 89-97. Date of Electronic Publication: 2017 Dec 14.
DOI: 10.1007/s11010-017-3254-z
Abstrakt: The present study is the first investigation of the inhibitory effect of Rhaponticum carthamoides transformed roots (TR) extract on the proliferation of grade II and III human glioma cells. TR extract showed the cytotoxic effect and inhibited the colony formation of both glioma cell lines in dose-dependent manner. The root extract induced apoptosis by increasing of the reactive oxygen species (about threefold compared to the control cells) leading to a disruption of mitochondrial membrane potential. Additionally, the mRNA levels of the apoptotic factors such as Bax, Tp53, caspase-3, and caspase-9 were observed to increase. These results indicate that the TR extract possesses anticancer activity by inhibiting glioma cell proliferation and inducing apoptotic cell death, and may be used as a promising anticancer agent.
Databáze: MEDLINE
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