Topical use and systemic action of green and roasted coffee oils and ground oils in a cutaneous incision model in rats (Rattus norvegicus albinus).

Autor: Lania BG; Laboratory of Applied Research in Dermatology and Bartonella Infection,-School of Medical Sciences-University of Campinas. Campinas, Sao Paulo, Brazil., Morari J; Cell Signaling Laboratory and Obesity and Comorbidities Research Center-School of Medical Sciences-University of Campinas. Campinas, Sao Paulo, Brazil., Souza AL; Metabolism Laboratory-Internal Medicine-School of Medical Sciences-University of Campinas. Campinas, Sao Paulo, Brazil., Silva MND; Laboratory of Applied Research in Dermatology and Bartonella Infection,-School of Medical Sciences-University of Campinas. Campinas, Sao Paulo, Brazil., de Almeida AR; Laboratory of Applied Research in Dermatology and Bartonella Infection,-School of Medical Sciences-University of Campinas. Campinas, Sao Paulo, Brazil., Veira-Damiani G; Laboratory of Applied Research in Dermatology and Bartonella Infection,-School of Medical Sciences-University of Campinas. Campinas, Sao Paulo, Brazil., Alegre SM; Metabolism Laboratory-Internal Medicine-School of Medical Sciences-University of Campinas. Campinas, Sao Paulo, Brazil.; Clinical Medicine Department-School of Medical Sciences-University of Campinas. Campinas, Sao Paulo, Brazil., César CL; National Institute of Photonics Applied to Cell Biology (INFABIC)-Gleb Wataghin Institute of Physics-University of Campinas. Campinas, Sao Paulo, Brazil., Velloso LA; Cell Signaling Laboratory and Obesity and Comorbidities Research Center-School of Medical Sciences-University of Campinas. Campinas, Sao Paulo, Brazil.; Clinical Medicine Department-School of Medical Sciences-University of Campinas. Campinas, Sao Paulo, Brazil., Cintra ML; Pathological Anatomy Department-School of Medical Sciences-University of Campinas. Campinas, Sao Paulo, Brazil., Maia NB; Aromatic Plants Division-Agronomic Institute of Campinas. Campinas, Sao Paulo, Brazil., Velho PENF; Laboratory of Applied Research in Dermatology and Bartonella Infection,-School of Medical Sciences-University of Campinas. Campinas, Sao Paulo, Brazil.; Clinical Medicine Department-School of Medical Sciences-University of Campinas. Campinas, Sao Paulo, Brazil.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2017 Dec 13; Vol. 12 (12), pp. e0188779. Date of Electronic Publication: 2017 Dec 13 (Print Publication: 2017).
DOI: 10.1371/journal.pone.0188779
Abstrakt: Introduction: Wounds are a common health problem. Coffee is widely consumed and its oil contains essential fatty acids. We evaluated the local (skin) and systemic effects associated with the topical use of coffee oils in rats.
Methods: Punch skin wounds (6 mm) incisions were generated on the backs of 75 rats. Saline (SS), mineral oil (MO), green coffee oil (GCO), roasted coffee oil (RCO), green coffee ground oil (GCGO) or roasted coffee ground oil (RCGO) were topically applied to the wounds. Healing was evaluated by visual and histological/morphometric optical microscopy examination; second harmonics generation (SHG) microscopy, wound tissue q-PCR (values in fold-change) and blood serum (ELISA, values in pg/mL).
Results: RCO treated animals presented faster wound healing (0.986 vs. 0.422), higher mRNA expression of IGF-1 (2.78 vs. 1.00, p = 0.01), IL-6 (10.72 vs. 1.00, p = 0.001) and IL-23 (4.10 vs. 1.2, p = 0.05) in early stages of wound healing; higher IL-12 (3.32 vs. 1.00, p = 0.05) in the later stages; and lower serum levels of IFN-γ (11.97 vs. 196.45, p = 0.01). GCO treatment led to higher mRNA expression of IL-6 (day 2: 7.94 vs. 1.00, p = 0.001 and day 4: 6.90 vs. 1.00, p = 0.01) and IL-23 (7.93 vs. 1.20, p = 0.001) in the early stages. The RCO treatment also produced higher serum IFN-α levels throughout the experiment (day 2: 52.53 vs. 21.20; day 4: 46.98 vs.21.56; day 10: 83.61 vs. 25.69, p = 0.05) and lower levels of IL-4 (day 4: 0.9 vs.13.36, p = 0.01), adiponectin (day 10: 8,367.47 vs. 16,526.38, p = 0.001) and IFN-γ (day 4: 43.03 vs.196.45, p = 0.05). The SHG analysis showed a higher collagen density in the RCO and GCO treatments (p = 0.05).
Conclusion: Topical treatment with coffee oils led to systemic actions and faster wound healing in rats. Further studies should be performed are necessary to assess the safety of topical vegetal oil use for skin lesions.
Databáze: MEDLINE
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